The experimental study of psychotropic and neurotropic properties of Acorus calamus leaves
Keywords:extract from Acorus calamus leaves, psychotropic properties, neurotropic properties, behavioral tests
One of the most widespread plants in Ukraine is sweet flag (Acorus calamus). Acorus calamus leaves possess the pharmacological properties. In recent years there have been data on the neurotropic properties of Acrous calamus extracts.
Aim. To determine the effect of Acorus calamus leaves on the animal’s behavioral responses, anxiety, depression, as well as the muscle tone and the coordination of movements.
Materials and methods. The dealcoholized water-alcohol extract of Acorus calamus leaves (conditional name ECL) was obtained by M.S. Yaremenko, a postgraduate student at the Department of Botany of the NUPh under the supervision of prof. T.M. Gontova. The psychotropic and neurotropic properties of the original extract from Acorus calamus leaves in the doses of 1 and 5 ml/kg by the behavioral responses of mice in the open field test, manifestations of depression in the tail suspension test, the course of thiopental-induced anesthesia and physical endurance in the forced swim test were studied.
Results. It has been found that the extract from Acorus calamus leaves has a dose-dependent effect on the locomotor, orienting-exploratory activity, as well as the muscle tone and the movement coordination of the experimental animals. In the doses of 1 and 5 ml/kg the extract from Acorus calamus leaves demonstrated a moderate actoprotective activity. The extract from Acorus calamus leaves in the dose of 5 ml/kg showed a moderate analeptic effect. The reference drug Bilobil in a single dose of 100 mg/kg did not show a significant effect on the behavioral responses, manifestations of depression, the course of thiopental-induced anesthesia and physical endurance of mice.
Conclusions. The extract from Acorus calamus leaves shows a dose-dependent effect on the animal’s behavioral responses, anxiety, depression, as well as the muscle tone and the coordination of movements. The extract from Acorus calamus leaves demonstrates a moderate actoprotective activity and a moderate analeptic effect. The data obtained indicate the necessity for further in-depth studies of extracts from Acorus calamus leaves to create effective drugs based on them for the correction of the central nervous system disorders.
Kovalov, V. M. (2000). Farmakohnoziia z osnovamy biokhimii roslyn. Kharkiv: Prapor, 167.
Harna, S. V., Vladymyrova, I. M., Burd, N. B., Heorhiiants, V. A., Kotov, A. H., Prokopenko, T. S., Vasylieva, O. A., ... Hlushchenko, A. V. (2016). Suchasna fitoterapiia. Navchalnyi posibnyk. Kharkiv: «Drukarnia Madryd», 580.
Hazra, R., Ray, K., & Guha, D. (2007). Inhibitory role of Acorus calamus in ferric chloride–induced epileptogenesis in rat. Human & Experimental Toxicology, 26(12), 947–953. https://doi.org/10.1177/0960327107087791
Pattanaik, J., Kumar, Y., Khatri, R. S. (2013). Acorus calamus Linn.: A herbal tonic for central nervous system. Journal of Scientific and Innovative Research, 2(5), 950–954.
Muthuraman, A., & Singh, N. (2012). Neuroprotective effect of saponin rich extract of Acorus calamus L. in rat model of chronic constriction injury (CCI) of sciatic nerve-induced neuropathic pain. Journal of Ethnopharmacology, 142(3), 723–731. https://doi.org/10.1016/j.jep.2012.05.049
Bhattacharyya, D, Sur, T. K., Lyle. N., Jana U, Debnath, P. K. (2011). A clinical study on the management of generalized anxiety disorder with Vaca (Acorus calamus). Ind J Trad Knowl, 10, 668–671.
Houghton, P. J., Kumar, V., Govindarajan, R., Mukherjee, P. K. (2006). Asarones in Acorus calamus and their acetylcholinesterase inhibition. J Pharm Pharmacol, 2, 52–55.
Patel, D. N., Ho, H. K., Tan, L. L., Tan, M.-M. B., Zhang, Q., Low, M.-Y., … Koh, H.-L. (2015). Hepatotoxic potential of asarones: in vitro evaluation of hepatotoxicity and quantitative determination in herbal products. Frontiers in Pharmacology, 6(25), 1–14. https://doi.org/10.3389/fphar.2015.00025
PDR for Herbal medicines (3rd ed.). (2004). Thomson Healthcare, 147–148.
Venskutonis, P. R., & Dagilyte, A. (2003). Composition of Essential Oil of Sweet Flag (Acorus calamusL.) Leaves at Different Growing Phases. Journal of Essential Oil Research, 15(5), 313–318. https://doi.org/10.1080/10412905.2003.9698598
Stefanov, O. V. (Ed.). (2001). Doklinichni doslidzhennia likarskykh zasobiv (metodychni rekomendatsii). Kyiv: «Avіcena», 528.
Rukovodstvo po provedeniiu doklinicheskikh issledovanii lekarstvennykh sredstv. (2012). Moscow: Grif i K, 944.
Tsyvunin, V. V., Shtryhol, S. Yu., Zahynaichenko, B. A. (2014). Pharmacology and Drug Toxicology, 2(38), 30–35.
Deiko, R. D., Shtryhol, S. Yu., Prusakov, A. N., Kolobovb, O. O. (2015). Ukraïns’kij bìofarmacevtičnij žurnal, 1(36), 14–20.
Porsolt, R. D., Lenegre, A., Elliot, J. M. (1992). Behavioral models of depression. Experimental Approaches to Anxiety and Depression. Chichester New York, 73–85.
Tarakhovskii, Iu. S. Kim, Iu. A., Abdrasilov, B. S., Muzafarov, E. N. (2013). Flavonoidy: biokhimiia, biofizika, meditcina. Pushchino: Sunchrobook, 310.
Nazarova, L. E. (2012). Issledovanie tcitoprotektornoi aktivnosti kisloty ferulovoi. Extended abstract of Doctor’s thesis. Pyatigorsk, 47.
Alekseeva, L. I., Bolotnik, E. V. (2013). Rastitelnyi mir aziatskoi Rossii, 1(11), 121–125.
Copyright (c) 2019 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).