The study of the acute toxicity in mice after administration of a phytosubstance glycosides of phenolic compounds from Vaccinum vitis-ideaea leaves in combination with arginine amino acid
Keywords:lead-compound, phytosubstance, Vaccinum vitis-idaea, acute toxicity
At the stage of the primary pharmacological screening the determination of the toxicity class of the phytosubstances with different qualitative and quantitative composition proposed as promising products for creating pharmacological medicines is the first step of preclinical studies, which purpose is to obtain results on toxicity and possible negative effects of the substance studied on the body of experimental animals in short-term effects of high doses of the drug.
Aim. To study the acute toxicity of the lead-compound in mice after intragastric administration.
Materials and methods. The object of the study was a modified neogalenical phytosubstance obtained from Vaccinum vitis-ideaea leaves. It is a complex of glycosides of phenolic compounds in combination with arginine amino acid. The acute toxicity was determined according to the methodological recommendations “Preclinical studies of medicinal products” edited by Stefanov O. V. on outbred mice weighing 20-26 g, adhering to the basic provisions of the Council of European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes.
Results. During the experimental study it was found that the lead-compound studied could be referred to relatively harmless substances (VI class according to Sydorov classification) as no signs of intoxication and lethality were detected when administering the intramuscular dose of 20000 mg/kg.
Conclusions. The acute toxicity of the lead-compound has been studied with intramuscular administration of the dose of 20000 mg/kg.
Malkoch, A. V., Filatova, N. N. Khasabov, N. N. (2016). Lechashchii vrach, 9, 7.
Erman, M. V. (2014). Effektivnaia farmakoterapiia. Pediatriia, 2.
Yusuke, N. (2008). Provizor, 2.
Tsemenko, K. V., Koshovyi, O. M., Kireiev, I. V., Komisarenko, M. A. (2006). Patent Ukrainy na korysnu model № 133723, UA, MPK (2006), A61K 31/05 (2006.01), A61K 127/00, A61P 13/00.
Tsemenko, K. V. (2018). Antibacterial activity of phytosubstants from Vaccinium vitis-idaea leaves. Annals of Mechnikov Institute, 3, 23–26. http://doi.org/10.5281/zenodo.1456526
6. Yevropeiska konventsiia pro zakhyst khrebetnykh tvaryn, shcho vykorystovuiutsia dlia doslidnykh ta inshykh naukovykh tsilei. (1986). 137. Available at: http://zakon.rada.gov.ua/laws/show/994_137
Stefanov, O. V. (2001). Doklinichni doslidzhennia likarskykh zasobiv (metodychni rekomendatsii). Kyiv: Avitsenna, 528.
Copyright (c) 2019 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).