The anti-apoptic action of the combination containing doxycycline and glucosamine in the experimental treatment of the joint syndrome
Keywords:doxycycline hydrochloride, glucosamine hydrochloride, combination, anti-apoptotic action, systemic steroid arthritis
Apoptosis is the main mechanism of chondrocytes death in osteoarthritis. Unlike necrosis, it is characterized as a process by which a cell itself actively initiates and causes its destruction. The following morphological and biochemical properties are characteristic for apoptosis: chromatin condensation and DNA fragmentation; the appearance of specific markers on the cell surface; cell shedding and the loss of contact with neighboring cells and the matrix; the nuclear membrane change; vacuolization and formation of apoptotic bodies. In the articular cartilage apoptosis occurs in response to compression, mechanical damage, disruption of trophism, or experimental influence.
Aim. To study the effect of the combination containing doxycycline and glucosamine on chondrocytes apoptosis processes on the model of systemic steroid arthrosis in rats compared to its active monocomponents – doxycycline and glucosamine.
Materials and methods. The therapeutic properties of the combination on the model of systemic steroid arthritis caused by dexamethasone were studied in-depth. It was reproduced by the intramuscular injection of dexamethasone phosphate three times in the dose of 7 mg/kg with an interval of one week with elements of modification consisted in increasing the dose of glucocorticosteroid. To assess apoptosis in an articular cartilage the TUNEL method was used.
Results. As a result of the morphometric studies a significant decrease in the number of TUNEL-positive apoptotic cells was observed under the influence of all the objects studied compared to the control pathology rats. It was also found that the combination studied had significant differences by this indicator compared to other experimental groups. Dexamethasone administration was shown to cause a proapoptotic effect. The composition has a protective effect on chondrocytes, balancing the processes of catabolism and anabolism of cellular homeostasis in them.
Conclusions. According to the results of the studies conducted the combination containing doxycycline and glucosamine can be assessed as an agent with a high chondroprotective activity; it is promising for further use in patients with destructive joint diseases, in particular osteoarthritis.
Kovalenko, V. M. (2012). Ukrainskyi revmatolohichnyi zhurnal, 49(3), 84–86.
Denison, H. J., Curtis, E. M., Clynes, M. A., Bromhead, C., Dennison, E. M., & Grainger, R. (2016). The incidence of sexually acquired reactive arthritis: a systematic literature review. Clinical Rheumatology, 35(11), 2639–2648. https://doi.org/10.1007/s10067-016-3364-0
Zeidler, H., & Hudson, A. (2016). Coinfection of Chlamydiae and other Bacteria in Reactive Arthritis and Spondyloarthritis: Need for Future Research. Microorganisms, 4(3), 30. https://doi.org/10.3390/microorganisms4030030
Molochkov, A. V., Molochkov, V. A., Petrova, M. S., Belousova, E. A., Mylov, N. M., Seliverstova, T. R., & Sekirin, A. B. (2016). A case of reactive post-enterocolitic arthritis (Reiter’s syndrome). Almanac of clinical medicine, (44-1), 45–51. https://doi.org/10.18786/2072-0505-2016-44-1-45-51
Molochkov, V. A., Kil’dyushevskiy, A. V., & Karzanov, O. V. (2016). Treatment of the tumor stage of mycosis fungoides with extracorporeal photochemotherapy (a case descript). Almanac of Clinical Medicine, (44-1), 103–106. https://doi.org/10.18786/2072-0505-2016-44-1-103-106
Borrelli, J., Tinsley, K., Ricci, W. M., Burns, M., Karl, I.E., & Hotchkiss, R. (2003). Induction of Chondrocyte Apoptosis Following Impact Load. Journal of Orthopaedic Trauma, 17(9), 635–641. https://doi.org/10.1097/00005131-200310000-00006
Huser, C. A. M., Peacock, M., & Davies, M. E. (2006). Inhibition of caspase-9 reduces chondrocyte apoptosis and proteoglycan loss following mechanical trauma. Osteoarthritis and Cartilage, 14(10), 1002–1010. https://doi.org/10.1016/j.joca.2006.03.012
Jin, CheolPark, Kyung, Taek Kim, Sung, Won Lee. (2005). Ad-TRAIL induces apoptosis in chondrocytes in vitro and neutralizing antibody to TRAIL prevents the іnduction of apoptosis in vitro and in vivo. The Korean J. Anat, 38(1), 63–71.
Zupanets, K. O., Shebeko, S. K., Otrishko, I.A. (2010). Liky Ukrainy plius, 3(12), 47−50.
Korzh, N. A., Dedukh, N. V., Zupanetc, I.A. (Eds.). (2007). Osteoartroz: konservativnaia terapiia: monografiia. Kharkiv, 424.
Zupanets, I. A., Tkachenko, K. M., Otrishko, I.A. (2014). Ukrainskyi zhurnal klinichnoi ta laboratornoi medytsyny, 9(3), 37–40.
12. European convention for the protection of vertebrate animals used for experimental and other scientific purpose: Council of Europe. (1986).Strasbourg, 52.
Hans-Jurgen Rode. (Ed.). (2008). Apoptosis, cytotoxicity and cell proliferation (4-th edition).Mannheim: Roche Diagnostics, 180.
Zupanets, I.A. (2015). Rozrobka efektyvnykh zasobiv protyzapalnoi ta khondroprotektornoi dii na osnovi tetratsykliniv ta hliukoza minu : inform. Lyst. Kyiv: Ukrmedpatentinform, 181–2015, 6.
Copyright (c) 2019 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).