The study of the anti-exudative action of the combined cream-gel with glucosamine hydrochloride, hondroitine sulfate, camphora and menthol in the experiment
Keywords:inflammation, cream-gel, glucosamine hydrochloride, anti-exudative action, carrageenin, zymosan, serotonin, histamine
Osteoarthritis (OA) is a severe degenerative-dystrophic joint disease. Treatment of OA should be complex with the use of painkillers, anti-inflammatory and chondroprotective agents. The original combined cream-gel with glucosamine hydrochloride, chondroitin sulfate, camphore and menthol meets these requirements.
Aim. To study the anti-exudative effect of the original combined cream-gel.
Materials and methods. The anti-exudative effect of the cream-gel was studied on the models of exudative inflammation caused by carrageenin (1 % solution), zymosan (2 %), histamine (0.25 %) and serotonin (0.5 %) in rats.
Results. The presence of the expressed anti-excudative effect on different inflammatory models by the level of antiinflammatory activity has been proven: carrageenan – 43.71 %, zymosanic – 32.83 %, serotonin – 24.04 %, histamine – 38.4 %. The results of studying the effect of the cream-gel under research on the inflammatory process with a predominantly exudative component on the models of acute carrageenan, zymosan, histamine, serotonin edema in experimental animals indicate the presence of an anti-exudative component in the cream-gel studied; it is somewhat inferior to the anti-inflammatory effect of diclofenac sodium. Based on the results of the experiments conducted it can be assumed that the anti-inflammatory effect is due to effect on the activity of pro-inflammatory biologically active substances – prostaglandins and leukotrienes, as well as the presence of membrane-stabilizing properties in the components of the cream-gel.
Conclusions. The presence of the anti-exudative action together with the data on the antinociceptive and chondroprotective action given in our previous works will promote to increase the effectiveness of treatment of such a serious joint disease as OA.
Korzh, N. A., Dedukh, N. V., Zupanetс, I. A. (2007). Osteoartroz: konservativnaia terapiia. Kharkov: Zolotye stranitcy, 424.
Hochberg, M., Chevalier, X., Henrotin, Y., Hunter, D. J., Uebelhart, D. (2013). Symptom and structure modification in osteoarthritis
with pharmaceutical–grade chondroitin sulfate: what’s the evidence? Current Medical Research and Opinion, 29 (3),
–267. doi: 10.1185/03007995.2012.753430
Wu, W., Pasierb, M. (2012). Physiological concentrations of glucosamine sulfate and glucosamine HCL can downregulate interleukin–
, KININS and MMPS in human osteoarthritic chondrocytes. Osteoarthritis and Cartilage, 20, S147. doi: 10.1016/j.
Pavlova, V. N., Pavlov, G. G., Shostak, N. A., Slutckii, L. I. (2011). Sustav: morfologiia, klinika, diagnostika, lechenie. Moscow:
Meditcinskoe informatcionnoe agentstvo, 397.
Jain, A., Mishra, S. K., Vuddanda, P. R., Singh, S. K., Singh, R., Singh, S. (2014). Targeting of diacerein loaded lipid nanoparticles
to intra–articular cartilage using chondroitin sulfate as homing carrier for treatment of osteoarthritis in rats. Nanomedicine:
Nanotechnology, Biology and Medicine, 10 (5), e1031–e1040. doi: 10.1016/j.nano.2014.01.008
Pulsatelli, L., Addimanda, O., Brusi, V., Pavloska, B., Meliconi, R. (2012). New findings in osteoarthritis pathogenesis: therapeutic
implications. Therapeutic Advances in Chronic Disease, 4 (1), 23–43. doi: 10.1177/2040622312462734
Stefanov, A. V. (2003). Doklіnіchnі doslіdzhennia lіkarskykh zasobіv. Kyiv: Avіtsena, 528.
Kennedy, O. D., Herman, B. C., Laudier, D. M., Majeska, R. J., Sun, H. B., Schaffler, M. B. (2012). Activation of resorption in
fatigue–loaded bone involves both apoptosis and active pro–osteoclastogenic signaling by distinct osteocyte populations.
Bone, 50 (5), 1115–1122. doi: 10.1016/j.bone.2012.01.025
Gadó, K., Gigler, G. (1991). Zymosan inflammation: A new method suitable for evaluating new antiinflammatory drugs. Agents
and Actions, 32 (1–2), 119–121. doi: 10.1007/bf01983335
Zupanetc, I. A., Korzh, N. A., Dedukh, N. V. et al. (1999). Metodicheskie rekomendatcii po eksperimentalnomu issledovaniiu i
klinicheskomu izucheniiu protivoartroznykh (khondromoduliruiushchikh) lekarstvennykh sredstv. Kyiv, 56.
Zupanetc, I. A., Zimin, S. M. (2013). European applied sciences, 1 (10), 48–51.
Shebeko, S. K., Zimin, S. M. (2016). The study of the effects of “Chondrolife” combined cream–gel in the spontaneous pain
sensitivity experiment. Clinical Pharmacy, 20 (3), 34–38.
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