The study of the anti-exudative action of the combined cream-gel with glucosamine hydrochloride, hondroitine sulfate, camphora and menthol in the experiment

S. M. Zimin, S. K. Shebeko, O. O. Lyapunova, T. S. Zhulai, O. O. Andrieieva

Abstract


Osteoarthritis (OA) is a severe degenerative-dystrophic joint disease. Treatment of OA should be complex with the use of painkillers, anti-inflammatory and chondroprotective agents. The original combined cream-gel with glucosamine hydrochloride, chondroitin sulfate, camphore and menthol meets these requirements.
Aim. To study the anti-exudative effect of the original combined cream-gel.
Materials and methods. The anti-exudative effect of the cream-gel was studied on the models of exudative inflammation caused by carrageenin (1 % solution), zymosan (2 %), histamine (0.25 %) and serotonin (0.5 %) in rats.
Results. The presence of the expressed anti-excudative effect on different inflammatory models by the level of antiinflammatory activity has been proven: carrageenan – 43.71 %, zymosanic – 32.83 %, serotonin – 24.04 %, histamine – 38.4 %. The results of studying the effect of the cream-gel under research on the inflammatory process with a predominantly exudative component on the models of acute carrageenan, zymosan, histamine, serotonin edema in experimental animals indicate the presence of an anti-exudative component in the cream-gel studied; it is somewhat inferior to the anti-inflammatory effect of diclofenac sodium. Based on the results of the experiments conducted it can be assumed that the anti-inflammatory effect is due to effect on the activity of pro-inflammatory biologically active substances – prostaglandins and leukotrienes, as well as the presence of membrane-stabilizing properties in the components of the cream-gel.
Conclusions. The presence of the anti-exudative action together with the data on the antinociceptive and chondroprotective action given in our previous works will promote to increase the effectiveness of treatment of such a serious joint disease as OA.


Keywords


inflammation; cream-gel; glucosamine hydrochloride; anti-exudative action; carrageenin; zymosan; serotonin; histamine

References


Korzh, N. A., Dedukh, N. V., Zupanetс, I. A. (2007). Osteoartroz: konservativnaia terapiia. Kharkov: Zolotye stranitcy, 424.

Hochberg, M., Chevalier, X., Henrotin, Y., Hunter, D. J., Uebelhart, D. (2013). Symptom and structure modification in osteoarthritis

with pharmaceutical–grade chondroitin sulfate: what’s the evidence? Current Medical Research and Opinion, 29 (3),

–267. doi: 10.1185/03007995.2012.753430

Wu, W., Pasierb, M. (2012). Physiological concentrations of glucosamine sulfate and glucosamine HCL can downregulate interleukin–

, KININS and MMPS in human osteoarthritic chondrocytes. Osteoarthritis and Cartilage, 20, S147. doi: 10.1016/j.

joca.2012.02.212

Pavlova, V. N., Pavlov, G. G., Shostak, N. A., Slutckii, L. I. (2011). Sustav: morfologiia, klinika, diagnostika, lechenie. Moscow:

Meditcinskoe informatcionnoe agentstvo, 397.

Jain, A., Mishra, S. K., Vuddanda, P. R., Singh, S. K., Singh, R., Singh, S. (2014). Targeting of diacerein loaded lipid nanoparticles

to intra–articular cartilage using chondroitin sulfate as homing carrier for treatment of osteoarthritis in rats. Nanomedicine:

Nanotechnology, Biology and Medicine, 10 (5), e1031–e1040. doi: 10.1016/j.nano.2014.01.008

Pulsatelli, L., Addimanda, O., Brusi, V., Pavloska, B., Meliconi, R. (2012). New findings in osteoarthritis pathogenesis: therapeutic

implications. Therapeutic Advances in Chronic Disease, 4 (1), 23–43. doi: 10.1177/2040622312462734

Stefanov, A. V. (2003). Doklіnіchnі doslіdzhennia lіkarskykh zasobіv. Kyiv: Avіtsena, 528.

Kennedy, O. D., Herman, B. C., Laudier, D. M., Majeska, R. J., Sun, H. B., Schaffler, M. B. (2012). Activation of resorption in

fatigue–loaded bone involves both apoptosis and active pro–osteoclastogenic signaling by distinct osteocyte populations.

Bone, 50 (5), 1115–1122. doi: 10.1016/j.bone.2012.01.025

Gadó, K., Gigler, G. (1991). Zymosan inflammation: A new method suitable for evaluating new antiinflammatory drugs. Agents

and Actions, 32 (1–2), 119–121. doi: 10.1007/bf01983335

Zupanetc, I. A., Korzh, N. A., Dedukh, N. V. et al. (1999). Metodicheskie rekomendatcii po eksperimentalnomu issledovaniiu i

klinicheskomu izucheniiu protivoartroznykh (khondromoduliruiushchikh) lekarstvennykh sredstv. Kyiv, 56.

Zupanetc, I. A., Zimin, S. M. (2013). European applied sciences, 1 (10), 48–51.

Shebeko, S. K., Zimin, S. M. (2016). The study of the effects of “Chondrolife” combined cream–gel in the spontaneous pain

sensitivity experiment. Clinical Pharmacy, 20 (3), 34–38.


GOST Style Citations


1. Остеоартроз: консервативная терапия : монография / под ред. Н. А. Коржа, Н. В. Дедух, И. А. Зупанца. – Харьков :
Золотые страницы, 2007. – 424 с.

2. Symptom and structure modification in osteoarthritis with pharmaceutical–grade chondroitin sulfate : what’s the evidence?
/ M. Hochberg, X. Chevalier, Y. Henrotin et al. // Curr. Med. Res. Opin. – 2013. – Vol. 29, Issue 3. – P. 259–267. doi:
10.1185/03007995.2012.753430

3. Wu, W. Physiological concentrations of glucosamine sulfate and glucosamine HCL can downregulate IL–1, kinins and MMPs
in human osteoarthritic chondrocytes / W. Wu, M. Pasierb // Osteoarthritis and Cartilage. – 2012. – Vol. 20. – S147 p. doi:
10.1016/j.joca.2012.02.212

4. Сустав : морфология, клиника, диагностика, лечение / под ред. В. Н. Павловой, Г. Г. Павлова, Н. А. Шостак, Л. И. Слуц-
кого. – М. : Медицинское информационное агентство, 2011. – 397 с.

5. Targeting of diacerein loaded lipid nanoparticles to intra–articular cartilage using chondroitin sulfate as homing carrier
for treatment of osteoarthritis in rats / A. Jain, S. K. Mishra, P. R. Vuddanda et al. // Nanomedicine. – 2014. – Vol. 10, Issue
5. – P. e1031–e1040. doi: 10.1016/j.nano.2014.01.008

6. New findings in osteoarthritis pathogenesis: therapeutic implications / L. Pulsatelli, O. Addimanda, V. Brusi et al. / Ther.
Adv. Chronic Dis. – 2012. – Vol. 4, Issue 1. – P. 23–43. doi: 10.1177/2040622312462734

7. Доклінічні дослідження лікарських засобів : метод. рек. / під ред. чл.-кор. НАМН Украины А. В. Стефанова. – К. :
Авіценна, 2003. – 528 с.

8. Kennedy, O. D. Activation of resorption in fatigue–loaded bone involves both apoptosis and active pro–osteoclastogenic
signaling by distinct osteocyte cell populations / O. D. Kennedy, B. C. Herman // Bone. – 2012. – Vol. 50, Issue 1. – P. 1115–1122.
doi: 10.1016/j.bone.2012.01.025

9. Gado, K. Zymosan inflammation : A new method suitable for evaluating new anti–inflammatory drugs / K. Gado, G. Gigler //
Agent and Actions. – 1991. – Vol. 32, Issue 1–2. – P. 119–121. doi: 10.1007/bf01983335

10. Методические рекомендации по экспериментальному исследованию и клиническому изучению противоартрозных
(хондромодулирующих) лекарственных средств / И. А. Зупанец, Н. А. Корж, Н. В. Дедух и др. – К., 1999. – 56 с.

11. Зупанец, И. А. Анализ эффективности оригинального комбинированного хондропротектора на модели системного
стероидного артроза у крыс / И. А. Зупанец, С. М. Зимин // Еur. Applied Sci. – 2013. – № 1 (10). – С. 48–51.

12. Zimin, S. M. The study of the effects of “Chondrolife” combined cream–gel in the spontaneous pain sensitivity experiment /
S. K. Shebeko, S. M. Zimin // Clinical Pharmacy. – 2016. – Vol. 20, Issue 3. – P. 34–38.





DOI: https://doi.org/10.24959/cphj.17.1432

Abbreviated key title: Klìn. farm.

ISSN 2518-1572 (Online), ISSN 1562-725X (Print)