DOI: https://doi.org/10.24959/cphj.20.1537

The screening study of the hypoglycemic activity of herbal mixtures (presentation 3)

A. O. Savych, S. M. Marchyshyn, I. I. Milian

Abstract


Diabetes mellitus is an important social and medical problem as it causes the development of dangerous complications leading to disability and mortality. This disease is characterized by a multi-vector pathogenesis that requires a comprehensive approach to the treatment. Due to the use of mixtures of medicinal plants in the treatment of diabetes it is possible to cover all aspects of the development of this disease and its complications.

Aim. To study the hypoglycemic activity of herbal mixtures used in folk medicine for the prevention and treatment of diabetes mellitus type 2, and determine their conditional therapeutic dose.

Materials and methods. The study was performed on male albino rats weighing 180-200 g. For the preventive treatment within 20 days they received orally the aqueous extracts (1:10) of the herbal mixtures studied in the doses of 6 mL/kg/day, 9 mL/kg/day and 12 mL/kg/day and the reference drugs – the official herbal mixture “Arfazetin” in the dose of 9 mL/kg/day and metformin tablets in the dose of 60 mg/kg/day. The study of hypoglycemic properties and determination of the conditional therapeutic dose of the mixtures studied were performed using glucose loading tests (oral glucose tolerance test – OGTT and intraperitoneal glucose tolerance test– IPGTT). All experiments were performed in accordance with general ethical principles with the recommendations of the EEC Council Directive 2010/63/EU about the protection of animals used for scientific purposes.

Results and discussion. The results of the study showed that the 20-day preventive treatment with the herbal mixtures reduced alimentary hyperglycemia 30 minutes after OGTT and regulated carbohydrate tolerance disorders by reducing hyperglycemia 15 minutes after IPGTT. The herbal mixture No. 13 in the dose of 12 mL/kg/day showed the highest hypoglycemic activity, which was almost similar with the reference drug – metformin tablets, but exceeded the official herbal mixture “Arfazetin” by its efficiency. In addition, the dose-dependence of the effectiveness of all five herbal mixtures studied was determined.

Conclusions. For the first time, the screening study of the hypoglycemic activity of herbal mixtures used in folk medicine for the prevention and treatment of diabetes mellitus type 2 has been conducted. It has been determined that the herbal mixture No. 13 containing Cichorii radices, Elymi repens rhizomata, Helichrysi arenarii flores, Rosae fructus, Maydis style cum stigmatis shows the highest effectiveness by the ability to reduce alimentary hyperglycemia during OGTT and reduce impaired carbohydrate tolerance during IPGTT. Its conditional therapeutic dose, which is 12 mL/kg/day, has been determined.


Keywords


herbal mixtures; hypoglycemic activity; diabetes mellitus; oral glucose tolerance test; intraperitoneal glucose tolerance test

Full Text:

PDF

References


American Diabetes Association. (2020). Standards of medical care in diabetes. Diabetes care, 43, 1212.

International Diabetes Federation. (2019). IDF Diabetes Atlas, 9th ed. Brussels, Available at: https://www.diabetesatlas.org

Skyler, J. S., Bakris, G. L., Bonifacio, E., Darsow, T., Eckel, R. H., Groop, L. et al. (2017). Differentiation of diabetes by pathophysiology, natural history, and prognosis. Diabetes, 66 (2), 241‐255. doi: https://doi.org/10.2337/db16-0806

Ndjaboue, R., Farhat, I., Ferlatte, C. A., Ngueta, G., Guay, D., Delorme, S. et al. (2020). Predictive models of diabetes complications: protocol for a scoping review. Systematic reviews, 9 (1), 137. doi: https://doi.org/10.1186/s13643-020-01391-w

Gothai, S., Ganesan, P., Park, S., Fakurazi, S., Choi, D., Arulselvan, P. (2016). Natural phyto-bioactive compounds for the treatment of type 2 diabetes: inflammation as a target. Nutrients, 8 (8), 461. doi: https://doi.org/10.3390/nu8080461

Governa, P., Baini, G., Borgonetti, V., Cettolin, G., Giachetti, D., Magnano, A. R. et al. (2018). Phytotherapy in the management of diabetes: a review. Molecules, 23 (1), 105. doi: https://doi.org/10.3390/molecules23010105

Kooti, W., Farokhipour, M., Asadzadeh, Z., Ashtary-Larky, D., Asadi-Samani, M. (2016). The role of medicinal plants in the treatment of diabetes: a systematic review. Electronic physician, 8 (1), 1832–1842. doi: https://doi.org/10.19082/1832

Savych, A. O., Marchyshyn, S. M., Kozyr, H. R., Skrinchuk, O. Y. (2019). Osnovni pryntsypy vykorystannya likarskykh roslyn ta yikh zboriv dlya likuvannya ta profilaktyky tsukrovoho diabetu 2 typu. Journal Phypotherapy, 4, 43-46. doi: https://doi.org/10.33617/2522-9680-2019-4-43

Savych, A., Marchyshyn, S., Basaraba, R., Lukanyuk, M. (2020). Antihyperglycemic, hypolipidemic and antioxidant properties of the herbal mixtures in dexamethasone-induced insulin resistant rats. PharmacologyOnLine, 2, 73-82.

Tovstuha, Ye. S. (2010). Zoloti retsepty ukrainskoi narodnoi medytsyny. Kyiv: Kraina Mriy Publishers, 550.

World Health Organization. (2003). WHO Guidelines on good agricultural and mixture practices (GACP) for medicinal plants. Geneva, Switzerland, 72. Available at: https://apps.who.int/iris/handle/10665/42783

EEC. (2010). «Council directive 2010/63/EU, of the 22nd September 2010 on the approximation of laws, regulations and administrative provisions of the member states regarding the protection of animals used for experimental and other scientific purposes». Offical Journal of the European Communities, 1–29. Available at: https://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:276:0033:0079:en:PDF

Savych, A., Marchyshyn, S. (2017). Investigation of pharmacological activity the new antidiabetic plant gathering in streptozotocin-nicotinamide-induced diabetes in the rats. The Pharma Innovation Journal, 6 (3), 175-177.

Horakova, O., Kroupova, P., Bardova, K., Janovska, P., Kopecky J. et al. (2019). Metformin acutely lowers blood glucose levels by inhibition of intestinal glucose transport. Scientific Reports, 9, 6156. doi: https://doi.org/10.1038/s41598-019-42531-0.

Stefanov, O. V. (2001). Doklinichni doslidzhennia likarskykh zasobiv. Kyiv: Avitsena Publishers, 528.

Kietsiriroje, N., Kanjanahirun, K., Kwankaew, J., Ponrak, R., Soonthornpun, S. (2018). Phytosterols and inulin-enriched soymilk increases glucagon-like peptide-1 secretion in healthy men: double-blind randomized controlled trial, subgroup study. BMC research notes, 11 (1), 844. doi: https://doi.org/10.1186/s13104-018-3958-5

Paternoster, S., Falasca, M. (2018). Dissecting the physiology and pathophysiology of glucagon-like peptide-1. Frontiers in Endocrinology, 9, 584. doi: https://doi.org/10.3389/fendo.2018.00584

Kang, G. G., Francis, N., Hill, R., Waters, D., Blanchard, C., Santhakumar, A. B. (2019). Dietary polyphenols and gene expression in molecular pathways associated with type 2 diabetes mellitus: A Review. International Journal of Molecular Sciences, 21 (1), 140. doi: https://doi.org/10.3390/ijms21010140

Adisakwattana, S. (2017). Cinnamic acid and its derivatives: mechanisms for prevention and management of diabetes and its complications. Nutrients, 9 (2), 163. doi: https://doi.org/10.3390/nu9020163

Sarian, M. N., Ahmed, Q. U., Mat So’ad, S. Z., Alhassan, A. M., Murugesu, S., Perumal, V. et al. (2017). Antioxidant and antidiabetic effects of flavonoids: a structure-activity relationship based study. BioMed research international, 2017, 14. doi: https://doi.org/10.1155/2017/8386065

Panche, A. N., Diwan, A. D., Chandra, S. R. (2016). Flavonoids: an overview. Journal of nutritional science, 5, e47. doi: https://doi.org/10.1017/jns.2016.41

Kaurinovic, B., Vastag, G. (2019). Flavonoids and phenolic acids as potential natural antioxidants, Antioxidants, Emad Shalaby, IntechOpen. doi: https://doi.org/10.5772/intechopen.83731


GOST Style Citations


1.   American Diabetes Association. Standards of medical care in diabetes. Diabetes care. 2020. № 43. 1212 с.

 

2.   International Diabetes Federation. IDF Diabetes Atlas. 9th ed. URL: https://www.diabetesatlas.org

 

3.   Differentiation of diabetes by pathophysiology, natural history, and prognosis / J. S. Skyler et al. Diabetes. 2017. № 66 (2). Р. 241–255. DOI: https://doi.org/10.2337/db16-0806

 

4.   Predictive models of diabetes complications: protocol for a scoping review / R. Ndjaboue et al. Systematic reviews. 2020. № 9 (1). Р. 137. DOI: https://doi.org/10.1186/s13643-020-01391-w

 

5.   Natural phyto-bioactive compounds for the treatment of type 2 diabetes : inflammation as a target / S. Gothai et al. Nutrients. 2016. № 8 (8). Р. 461. DOI: https://doi.org/10.3390/nu8080461

 

6.   Phytotherapy in the management of diabetes: a review / P. Governa et al. Molecules. 2018. № 23 (1). Р. 105. DOI: https://doi.org/10.3390/molecules23010105

 

7.   The role of medicinal plants in the treatment of diabetes: a systematic review / W. Kooti et al. Electronic physician. 2016. № 8 (1). Р. 1832–1842. DOI: https://doi.org/10.19082/1832

 

8.   Савич А. О., Марчишин С. М., Козяр Г. Р., Скринчук О. Я. Основні принципи використання лікарських рослин та їх зборів для лікування та профілактики цукрового діабету 2 типу : огляд літератури. Фітотерапія часопис. 2019. № 4. С. 43–46.

 

9.   Savych А., Marchyshyn М., Basaraba R., Lukanyuk М. Antihyperglycemic, hypolipidemic and antioxidant properties of the herbal mixtures in dexamethasone-induced insulin resistant rats. Pharmacologyonline. 2020. № 2. P. 73–82.

 

10. Товстуха Є. С. Золоті рецепти української народної медицини. Київ : КМ-Букс, 2010. 550 c.

 

11. WHO Guidelines on good agricultural and mixture practices (GACP) for medicinal plants / World Health Organization. 2003. 72 р. URL: https://apps.who.int/iris/handle/10665/42783

 

12. EEC «Council directive 2010/63/EU, of the 22nd September 2010 on the approximation of laws, regulations and administrative provisions of the member states regarding the protection of animals used for experimental and other scientific purposes». Offical Journal of the European Communities. 2010. Р. 1–29. URL: https://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:276:0033:0079:en:PDF

 

13. Savych A., Marchyshyn S. Investigation of pharmacological activity the new antidiabetic plant gathering in streptozotocin-nicotinamide-induced diabetes in the rat. The Pharma Innovation Journal. 2017. № 6 (3). Р.175–177. URL: http://www.thepharmajournal.com/archives/?year=2017&vol=6&issue=3&ArticleId=995

 

14. Metformin acutely lowers blood glucose levels by inhibition of intestinal glucose transport / O. Horakova et al. Scientific Reports. 2019. № 9. Р. 61–56. DOI: https://doi.org/10.1038/s41598-019-42531-0

 

15. Доклінічні дослідження лікарських засобів : метод. рек. / за ред. чл.-кор. АМН України О. В. Стефанова. Київ : Авіценна, 2001. 528 с.

 

16. Phytosterols and inulin-enriched soymilk increases glucagon-like peptide-1 secretion in healthy men: double-blind randomized controlled trial, subgroup study / N. Kietsiriroje et al. BMC research notes. 2018. № 11 (1). Р. 844. DOI: https://doi.org/10.1186/s13104-018-3958-5

 

17. Paternoster S., Falasca M. Dissecting the physiology and pathophysiology of glucagon-like peptide-1. Frontiers in Endocrinology. 2018. № 9. Р. 584. DOI: https://doi.org/10.3389/fendo.2018.00584

 

18. Dietary polyphenols and gene expression in molecular pathways associated with type 2 diabetes mellitus: A Review / G. G. Kang et al. International Journal of Molecular Sciences. 2019. № 21 (1). Р. 140. DOI: https://doi.org/10.3390/ijms21010140

 

19. Adisakwattana S. Cinnamic acid and its derivatives: mechanisms for prevention and management of diabetes and its complications. Nutrients. 2017. № 9 (2). Р. 163. DOI: https://doi.org/10.3390/nu9020163

 

20. Antioxidant and antidiabetic effects of flavonoids: a structure-activity relationship based study / M. N. Sarian et al. BioMed research international. 2017. Vol. 2017. 14 р. DOI: https://doi.org/10.1155/2017/8386065

 

21. Panche A. N., Diwan A. D., Chandra S. R. Flavonoids: an overview. Journal of nutritional science. 2016. № 5. e47. DOI: https://doi.org/10.1017/jns.2016.41

 

22. Kaurinovic B., Vastag G. Flavonoids and phenolic acids as potential natural antioxidants. IntechOpen. 2019. DOI: https://doi.org/10.5772/intechopen.83731





Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Abbreviated key title: Clin. pharm.

ISSN 2518-1572 (Online), ISSN 1562-725X (Print)