The screening study of the hypoglycemic activity of herbal mixtures (presentation 3)




herbal mixtures, hypoglycemic activity, diabetes mellitus, oral glucose tolerance test, intraperitoneal glucose tolerance test


Diabetes mellitus is an important social and medical problem as it causes the development of dangerous complications leading to disability and mortality. This disease is characterized by a multi-vector pathogenesis that requires a comprehensive approach to the treatment. Due to the use of mixtures of medicinal plants in the treatment of diabetes it is possible to cover all aspects of the development of this disease and its complications.

Aim. To study the hypoglycemic activity of herbal mixtures used in folk medicine for the prevention and treatment of diabetes mellitus type 2, and determine their conditional therapeutic dose.

Materials and methods. The study was performed on male albino rats weighing 180-200 g. For the preventive treatment within 20 days they received orally the aqueous extracts (1:10) of the herbal mixtures studied in the doses of 6 mL/kg/day, 9 mL/kg/day and 12 mL/kg/day and the reference drugs – the official herbal mixture “Arfazetin” in the dose of 9 mL/kg/day and metformin tablets in the dose of 60 mg/kg/day. The study of hypoglycemic properties and determination of the conditional therapeutic dose of the mixtures studied were performed using glucose loading tests (oral glucose tolerance test – OGTT and intraperitoneal glucose tolerance test– IPGTT). All experiments were performed in accordance with general ethical principles with the recommendations of the EEC Council Directive 2010/63/EU about the protection of animals used for scientific purposes.

Results and discussion. The results of the study showed that the 20-day preventive treatment with the herbal mixtures reduced alimentary hyperglycemia 30 minutes after OGTT and regulated carbohydrate tolerance disorders by reducing hyperglycemia 15 minutes after IPGTT. The herbal mixture No. 13 in the dose of 12 mL/kg/day showed the highest hypoglycemic activity, which was almost similar with the reference drug – metformin tablets, but exceeded the official herbal mixture “Arfazetin” by its efficiency. In addition, the dose-dependence of the effectiveness of all five herbal mixtures studied was determined.

Conclusions. For the first time, the screening study of the hypoglycemic activity of herbal mixtures used in folk medicine for the prevention and treatment of diabetes mellitus type 2 has been conducted. It has been determined that the herbal mixture No. 13 containing Cichorii radices, Elymi repens rhizomata, Helichrysi arenarii flores, Rosae fructus, Maydis style cum stigmatis shows the highest effectiveness by the ability to reduce alimentary hyperglycemia during OGTT and reduce impaired carbohydrate tolerance during IPGTT. Its conditional therapeutic dose, which is 12 mL/kg/day, has been determined.

Author Biographies

A. O. Savych, I. Horbachevsky Ternopil National Medical University

Candidate of Pharmacy (Ph.D.), teaching assistant of the Department of Pharnacognosy with Medical Botany

S. M. Marchyshyn, I. Horbachevsky Ternopil National Medical University

Doctor of Pharmacy (Dr.habil.), professor, head of the Department of Pharnacognosy with Medical Botany

I. I. Milian, I. Horbachevsky Ternopil National Medical University

Candidate of Pharmacy (Ph.D.), teaching assistant of the Department of General Chemistry


American Diabetes Association. (2020). Standards of medical care in diabetes. Diabetes care, 43, 1212.

International Diabetes Federation. (2019). IDF Diabetes Atlas, 9th ed. Brussels, Available at:

Skyler, J. S., Bakris, G. L., Bonifacio, E., Darsow, T., Eckel, R. H., Groop, L. et al. (2017). Differentiation of diabetes by pathophysiology, natural history, and prognosis. Diabetes, 66 (2), 241‐255. doi:

Ndjaboue, R., Farhat, I., Ferlatte, C. A., Ngueta, G., Guay, D., Delorme, S. et al. (2020). Predictive models of diabetes complications: protocol for a scoping review. Systematic reviews, 9 (1), 137. doi:

Gothai, S., Ganesan, P., Park, S., Fakurazi, S., Choi, D., Arulselvan, P. (2016). Natural phyto-bioactive compounds for the treatment of type 2 diabetes: inflammation as a target. Nutrients, 8 (8), 461. doi:

Governa, P., Baini, G., Borgonetti, V., Cettolin, G., Giachetti, D., Magnano, A. R. et al. (2018). Phytotherapy in the management of diabetes: a review. Molecules, 23 (1), 105. doi:

Kooti, W., Farokhipour, M., Asadzadeh, Z., Ashtary-Larky, D., Asadi-Samani, M. (2016). The role of medicinal plants in the treatment of diabetes: a systematic review. Electronic physician, 8 (1), 1832–1842. doi:

Savych, A. O., Marchyshyn, S. M., Kozyr, H. R., Skrinchuk, O. Y. (2019). Osnovni pryntsypy vykorystannya likarskykh roslyn ta yikh zboriv dlya likuvannya ta profilaktyky tsukrovoho diabetu 2 typu. Journal Phypotherapy, 4, 43-46. doi:

Savych, A., Marchyshyn, S., Basaraba, R., Lukanyuk, M. (2020). Antihyperglycemic, hypolipidemic and antioxidant properties of the herbal mixtures in dexamethasone-induced insulin resistant rats. PharmacologyOnLine, 2, 73-82.

Tovstuha, Ye. S. (2010). Zoloti retsepty ukrainskoi narodnoi medytsyny. Kyiv: Kraina Mriy Publishers, 550.

World Health Organization. (2003). WHO Guidelines on good agricultural and mixture practices (GACP) for medicinal plants. Geneva, Switzerland, 72. Available at:

EEC. (2010). «Council directive 2010/63/EU, of the 22nd September 2010 on the approximation of laws, regulations and administrative provisions of the member states regarding the protection of animals used for experimental and other scientific purposes». Offical Journal of the European Communities, 1–29. Available at:

Savych, A., Marchyshyn, S. (2017). Investigation of pharmacological activity the new antidiabetic plant gathering in streptozotocin-nicotinamide-induced diabetes in the rats. The Pharma Innovation Journal, 6 (3), 175-177.

Horakova, O., Kroupova, P., Bardova, K., Janovska, P., Kopecky J. et al. (2019). Metformin acutely lowers blood glucose levels by inhibition of intestinal glucose transport. Scientific Reports, 9, 6156. doi:

Stefanov, O. V. (2001). Doklinichni doslidzhennia likarskykh zasobiv. Kyiv: Avitsena Publishers, 528.

Kietsiriroje, N., Kanjanahirun, K., Kwankaew, J., Ponrak, R., Soonthornpun, S. (2018). Phytosterols and inulin-enriched soymilk increases glucagon-like peptide-1 secretion in healthy men: double-blind randomized controlled trial, subgroup study. BMC research notes, 11 (1), 844. doi:

Paternoster, S., Falasca, M. (2018). Dissecting the physiology and pathophysiology of glucagon-like peptide-1. Frontiers in Endocrinology, 9, 584. doi:

Kang, G. G., Francis, N., Hill, R., Waters, D., Blanchard, C., Santhakumar, A. B. (2019). Dietary polyphenols and gene expression in molecular pathways associated with type 2 diabetes mellitus: A Review. International Journal of Molecular Sciences, 21 (1), 140. doi:

Adisakwattana, S. (2017). Cinnamic acid and its derivatives: mechanisms for prevention and management of diabetes and its complications. Nutrients, 9 (2), 163. doi:

Sarian, M. N., Ahmed, Q. U., Mat So’ad, S. Z., Alhassan, A. M., Murugesu, S., Perumal, V. et al. (2017). Antioxidant and antidiabetic effects of flavonoids: a structure-activity relationship based study. BioMed research international, 2017, 14. doi:

Panche, A. N., Diwan, A. D., Chandra, S. R. (2016). Flavonoids: an overview. Journal of nutritional science, 5, e47. doi:

Kaurinovic, B., Vastag, G. (2019). Flavonoids and phenolic acids as potential natural antioxidants, Antioxidants, Emad Shalaby, IntechOpen. doi:






Pre-clinical studies of new drugs