DOI: https://doi.org/10.24959/cphj.20.1533

Screening of the antioxidant activity of extracts from Hedera helix leaves using the HPLC/ABTS method

I. V. Bezruk, V. O. Grudko, V. A. Georgiyants, L. Ivanauskas

Abstract


Hedera helix is widely used as a remedy to treat the respiratory infections and cold accompanied with cough due to its anti-inflammatory effect. In addition, the antioxidant activity of its extracts has been confirmed. It is explained by the high content of flavonoids and phenolic acids among all phytochemicals of H. helix leaves. However, it remains uncertain which exactly components are responsible for the antioxidant activity and what is the best way to perform extraction.

Aim. To determine the antioxidant profile of different extracts from H. helix leaves using the in vitro HPLC method combined with the ABTS reagent.

Materials and methods. Extraction of H. helix leaves was conducted with different solvents (from 20 % methanol to 100 % methanol using an ultrasound bath); the method described in the Pharmacopeia was also used. A Waters chromatograph was used to determine the antioxidant profile.

Results. About 90 % of the components responsible for the antioxidant activity were determined using the HPLC method proposed. Among them, chlorogenic acid and 3,5-caffeoylquinic acid showed the highest activities. Other components, such as neochlorogenic acid, hyperoside and 3,4-caffeoylquinic acid were revealed as components with the antioxidant scavenging activities in H. helix lextracts.

Conclusions. The results obtained indicate that extracts from H. helix leaves possess the high antioxidant scavenging capacity. In addition, the in vitro HPLC method proposed can be used for the primary screening of components in the plant raw material.

Keywords


Hedera helix leaves; antioxidant activity; HPLC; screening

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References


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Marksa, M., Zymone K., Ivanauskas L., Radušienė J., Pukalskas A., Raudone L. (2020). Antioxidant profiles of leaves and inflorescences of native, invasive and hybrid Solidago species. Industrial Crops and Products, 145. doi: https://doi.org/10.1016/j.indcrop.2020.112123.

Raudonis R., Bumblauskiene L., Jakstas V., Pukalskas A., Janulis V. (2010). Optimization and validation of post-column assay for screening of radical scavengers in herbal raw materials and herbal preparations. J. Chromatogr. A., 1217, 7690-7698. doi: https://doi.org/10.1016/j.chroma.2010.10.017.


GOST Style Citations


1.   Obodozie O. O. Pharmacokinetics and drug interactions of herbal medicines: a missing critical step in the phytomedicine/drug development process.Readingsin Advanced Pharmacokinetics-Theory, Methods, and Applications. InTech. 2012. DOI: https://doi.org/10.5772/33699 (Date of access: 24.05.2020).

 

2.   Süleyman H., Mshvildadze V., Gepdiremen A., Elias R. Acute and chronic antiinflammatory profile of the ivy plant, Hedera helix, in rats. Phytomedicine. 2003. Vol. 10, № 5. P. 370-374. DOI: 10.1078/0944-7113-00260 (Date of access: 24.05.2020).

 

3.   Medeiros JMR, Macedo M., Contancia J.P., Nguyen C., Cunningham G., Miles D. H. Antithrombin activity of medicinal plants of the Azores. J Ethnopharmacol. 2000. Vol. 72, № 1-2. P. 157-165. DOI: 10.1016/s0378-8741(00)00226-9 (Date of access: 24.05.2020).

 

4.   Bezruk I., Kotvitska A., Korzh Iu., Materiienko A., Gubar S., Budanova L., Ivanauskas L., Vyshnevsky I., Georgiyants V. Combined Approach to the Choice of Chromatographic Methods for Routine Determination of Hederacoside C in Ivy Leaf Extracts, Capsules, and Syrup. Sci. Pharm. 2020. Vol. 88, № 24. DOI: https://doi.org/10.3390/scipharm88020024 (Date of access: 24.05.2020).

 

5.   Directive 2010/63/EU Of The European Parliament And Of The Council of 22 September 2010 on the protection of animals used for scientific purposes. URL: https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32010L0063&from=en (Date of access: 24.05.2020).

 

6.   Kolodeznaya T., Dobrova V. Ye. Alternative approaches of pathology’s modeling in pharmacological studies of medicines. Actual Questions Of Development оf New Drugs : Abstracts оf XX International Scientific And Practical Conference Of Young Scientists And Student, April 22-23, 2014. – Kharkiv : Publishing Office, 2014. P. 208.

 

7.   Bezruk I., Marksa M., Georgiyants V., Ivanauskas L., Raudone L. Phytogeographical profiling of ivy leaf (Hedera helix L.). Industrial Crops and Products. 2020. Vol. 154. DOI: https://doi.org/10.1016/j.indcrop.2020.112713 (Date of access: 24.05.2020).

 

8.   European Pharmacopoeia / European Directorate for the Quality of Medicines and HealthCare. 10th ed.Strasbourg, 2019.

 

9.   Marksa M., Radušienė J., Jakštas V., Ivanauskas L. Development of an HPLC post-column antioxidant assay for Solidagocanadensis radical scavengers. Natural Product Research. 2016. Vol. 30, Issue 5. P. 536-543. DOI: https://doi.org/10.1080/14786419.2015.1027703 (Date of access: 24.05.2020).

 

10. Marksa, M., Zymone K., Ivanauskas L., Radušienė J., Pukalskas A., Raudone L. Antioxidant profiles of leaves and inflorescences of native, invasive and hybrid Solidago species. Industrial Crops and Products. 2020. Vol. 145. DOI: https://doi.org/10.1016/j.indcrop.2020.112123 (Date of access: 24.05.2020).

 

11. Raudonis R., Bumblauskiene L., Jakstas V., Pukalskas A., Janulis V. Optimization and validation of post-column assay for screening of radical scavengers in herbal raw materials and herbal preparations. J. Chromatogr. A. 2010. Vol. 1217. P. 7690–7698. DOI: https://doi.org/10.1016/j.chroma.2010.10.017 (Date of access: 24.05.2020).





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Abbreviated key title: Clin. pharm.

ISSN 2518-1572 (Online), ISSN 1562-725X (Print)