The study of the hypoglycemic action of extracts from bearberry leaves under experimental insulin resistance in rats
Keywords:insulin resistance, bearberry, oral glucose tolerance test, diabetes mellitus type 2, hypoglycemic action
Type 2 diabetes mellitus (DM2) has been increasing steadily all over the world. A large number of medicinal plants that have the hypoglycemic effect are known, but, unfortunately, currently there is very limited choice of antidiabetic herbal medicines.
Aim. To design the experiment in order to study the hypoglycemic effect of polyphenolic extracts from bearberry (Arctostaphylos uva-ursi) leaves under the experimental insulin resistance (IR) in rats.
Materials and methods. The experimental IR was induced by dexamethasone injections (Dex) and feeding with a high-fructose diet (HFD). Male outbred albino rats were randomized depending on the purpose of the experiment. As the study objects 50 % ethanolic polyphenol extracts obtained from bearberry leaves with addition of arginine (PE50_arg) and cysteine (PE50_cys) were selected. The oral glucose tolerance test (OGTT) was performed in all experimental groups of animals.
Results. Dex had a more pronounced effect on tolerance to glucose compared to the HFD. It was shown that PE50_arg and PE50_cys after two weeks of administration revealed the ability to decrease the blood glucose level in rats, as well as reduce IR development and improve tolerance to glucose under the experimental IR. The hypoglycemic activity found did not much differ from the action of Metformin, but exceeded the activity of Arphazetin. These results can be the evidence of activation of glucose utilization processes, and it, in turn, indicates the insulin sensitivity improvement due to the action of the extracts studied. The data obtained indicate that the corrective effect of arginine and cysteine on signal transduction processes in insulin target cells plays an important role in the IR treatment.Conclusions. Bearberry leaves are the promising raw material for creating an anti-diabetic drug. Thus, it is necessary to further study the mechanisms of regulation of metabolic disorders when introducing new polyphenolic extracts.
Khawandanah, J. (2019). Double or hybrid diabetes: A systematic review on disease prevalence, characteristics and risk factors. Nutrition & Diabetes, 9 (1). doi: https://doi.org/10.1038/s41387-019-0101-1.
Nolan, C. J., Prentki, M. (2019). Insulin resistance and insulin hypersecretion in the metabolic syndrome and type 2 diabetes: Time for a conceptual framework shift. Diabetes & Vascular Disease Research, 16 (2), 118–127. doi: https://doi.org/10.1177/1479164119827611.
Chaimum-aom, N., Chomko, S., Talubmook, C. (2017). Toxicology and Oral Glucose Tolerance Test (OGTT) of Thai Medicinal Plant Used for Diabetes control, Phyllanthus acidus L. (EUPHORBIACEAE). Pharmacognosy Journal, 9 (1), 58–61. doi: https://doi.org/10.5530/pj.2017.1.11.
Missoun, F., Bouabdelli, F., Baghdad, A., Amari, N., Djebli, N. (2018). Antidiabetic bioactive compounds from plants. Medical Technologies Journal, 2 (2), 199–214. doi: https://doi.org/10.26415/2572-004x-vol2iss2p199-214.
Zahaiko, A. L., Briukhanova, T. O., Shkapo, A. I. (2015). Informatsiinyi lyst Ukrmedpatentinformu pro novovvedennia v systemi Okhorony zdorov’ia (Vol. 86). Kyiv.
Kravchenko, G., Krasilnikova, O., Mazen, M. (2020) Hypoglycemic and hypolipidemic activity of arginine containing bearberry leaves extract in insulin resistant rats. Medychna ta klinichna khimiya, 1, 5-10. doi: https://doi.org/10.24959/ubphj.18.173.
Kravchenko, G., Mazen, M., Krasilnikova, O. (2018). Screening of Bearberry leaves extracts hypoglycemic effect and study of acute toxicity. Ukrainian biopharmaceutical journal, 55 (2), 13–16. doi: https://doi.org/10.24959/ubphj.18.173.
Suman, R. K., Mohanty, I.R., Maheshwari, U., Borde, M. K., Deshmukh, Y. A. (2016). Metformin ameliorates diabetes with metabolic syndrome induced changes in experimental rats. International Journal of Biomedical Research, 7 (2), 55–65. doi: https://doi.org/10.7439/ijbr.v7i2.2987.
Stefanov, O. V. (2001). Doklіnichni doslidzhennia likarskykh zasobiv. Kyiv: Avitsena, 528.
de Oliveira, D. T., Souza–Silva, E., Tonussi, C. R. (2009). Gingival Vein Punction: A New Simple Technique for Drug Administration or Blood Sampling in Rats and Mice. Scandinavian Journal of Laboratory Animal Science, 36 (2), 109–113.
Sakaguchi, K., Takeda, K., Maeda, M., Ogawa, W., Sato, T., Okada, S., … Kashiwagi, A. (2015). Glucose area under the curve during oral glucose tolerance test as an index of glucose intolerance. Diabetology International, 7 (1), 53–58. doi: https://doi.org/10.1007/s13340-015-0212-4.
Copyright (c) 2020 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).