The study of the pharmacological action of the dry extract from Prunus domestica fruits on the model of comorbid functional constipation in the combined alcoholic liver damage in rats
DOI:
https://doi.org/10.24959/167342Keywords:
Prunes Domestica fruits, hepatoprotectors, comorbid fuctional constipation, subacute liver damage, urea, ALTAbstract
The search for the most effective hepatoprotective agents is an urgent problem of modern medicine. As a rule, crude plant is promising object for the study of medicinal properties. Based on the literature data, we are interested in the horticultural crops Prunus domestica, the family Rosaceae, which is quite cultivated and attracts attention to its pharmacoeconomic component.
The purpose of the study. In previous experimental studies, the Prunus domestica extract, containing fibers, was found to have laxative, hepatoprojector and probiotic effects. The foregoing provided an opportunity to study the Prunus domestica extract, containing fibers for activity in the comorbidization of several relevant pathologies and to experimentally substantiate the prospect of its application as a means of choice for constipation in patients with pathologies of the digestive system.
Materials and methods. The object of research was a dry extract from Prunus domestica fruits, containing fibers. The experiment was carried out in stages. At the first stage, the animals caused experimental subacute alcoholic liver damage. In the second stage of the experiment, the animals were modeled with functional constipation. During the course of subacute hepatosis and during the second phase of the experiment, the test extract was injected into the animal at a dose of 200 mg/kg and the reference drug was hepatoprotector Silybor at a dose of 25 mg/kg. In the second phase of the study, the laxative agent Senadexin at a dose of 14 mg/kg was added to the therapy. All substances were injected into the body of the experimental animals orally.
To evaluate the motor activity of the intestine, Sagar et al, modified by Choi et al., was used.
Motor activity of the small intestine was determined according to the corresponding calculation formula.
The functional state of the liver was evaluated according to the biochemical parameters in the blood serum: the total protein, urea and ALT activity.
Results. An analysis of experimental data showed that the test extract exhibits a moderate laxative effect.
In animals of the intact group, the relevant signs (pronounced laxative effect) indicate the development of diarrhea, which is not an adequate solution to the development of constipation.
With the use of the extract from Prunus domestica fruits during the whole period of the experiment, normalization of all indicators that characterize the intestinal motility was observed.
Analysis of experimental data showed that the investigated phytoobject has a soft laxative effect, mainly through the improvement of intestinal motility.
In the study of the functional state of the liver on the introduction of the investigated extract, positive changes in the content of markers of liver damage (decrease in urea concentration and decrease in the activity of ALT in animal serum) were detected in comparison with the control group of animals.
Conclusions. The obtained experimental data on the study of the correlation mechanisms of hepatoprotective and laxative activity of the extract from Prunus domestica fruits containing fibers indicate that there is a soft laxative effect that is realized with the help of intensifying intestinal motility, as well as the presence of pronounced hepatoprotective action. The indicated effects of the investigated extract were revealed when used in the treatment of constipation on experimental subacute liver damage, while not losing out to the studied effects in the complex application of hepatoprotective and laxative comparisons.
The key conclusion is that the test extract, as opposed to the reference drug, does not cause signs of diarrhea in its laxative effect, which can be a distinctive, positive feature in further clinical studies.
This herbal object can be promising and rational in application with single-stage complex therapy, such as hepatoprotective and laxative agent in the treatment of comorbid conditions in gastroenterology associated with diseases of the liver and congestive intestinal phenomena.
References
Garny`k, T. P. (2008). Fitoterapiya. Chasopy`s, 1, 59–63.
Zhou, Y., Li, Y., Zhou, T., Zheng, J., Li, S., & Li, H.-B. (2016). Dietary Natural Products for Prevention and Treatment of Liver Cancer. Nutrients, 8(3), 156. https://doi.org/10.3390/nu8030156
Bose, M., Kamra, M., Mullick, R., Bhattacharya, S., Das, S., & Karande, A. A. (2017). Identification of a flavonoid isolated from plum (Prunus domestica) as a potent inhibitor of Hepatitis C virus entry. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-04358-5
Upy`r, L. V. (2010). Sly`va: Farmacevty`chna ency`klopediya. Kyiv: Morion, 1290.
Mohammed Shahm Basim, Lenchik, L. V., Caidov, N. B. (2017). (Prunus Domestica fruits – perspective row for drugs development. Nauka i innovaciya. Seriya estestvennyh nauk, 4, 42–48.
Upyr, T., Basim Mohammed, S., Bashar, A.-J. A. S., Lenchyk, L., Senyuk, I., & Kyslychenko, V. (2018). Phytochemical and pharmacological study of polysaccharide complexes of prunus domestica fruit. ScienceRise: Pharmaceutical Science, 3 (13), 32–37. https://doi.org/10.15587/2519-4852.2018.135825
Celik, F. (2017). Determination of phenolic compounds, antioxidant capacity and organic acids contents of Prunus domestica L., Prunus cerasifera Ehrh. and Prunus spinosa L. fruits by HPLC. Acta Chromatographica, 29(4), 507–510. http://doi.org/10.1556/1326.2017.00327.
Senyuk, I., Bashar, A., & Lenchyk, L. (2017). Investigation of different substances catharic properties made from Prunus domestica. UkraïNs’Kij BìOfarmacevtičNij žUrnal, 5(52), 21–25. https://doi.org/10.24959/ubphj.17.134
Senjuk, I. V., Al Sahlani, B. J., & Basim, M. S. (2018). Farmacevty`chny`j chasopy`s, 4, 57–61. https://doi.org/10.11603/2312-0967.2018.4.9692
Kalaitzakis, E. (2014). Gastrointestinal dysfunction in liver cirrhosis. World Journal of Gastroenterology, 20(40), 14686. https://doi.org/10.3748/wjg.v20.i40.14686
Scalera, A., Di Minno, M. N., Tarantino, G. (2013). What does irritable bowel syndrome share with non-alcoholic fatty liver disease? World journal of gastroenterology, 19(33), 5402–5420. https://doi.org/10.3748/wjg.v19.i33.5402
Yoon, E., Babar, A., Choudhary, M., Kutner, M., Pyrsopoulos, N. (2016). Acetaminophen-Induced Hepatotoxicity: a Comprehensive Update. Journal of clinical and translational hepatology, 4 (2), 131–142. https://doi.org/10.14218/jcth.2015.00052
Reding, K. W., Cain, K. C., Jarrett, M. E., Eugenio, M. D., & Heitkemper, M. M. (2013). Relationship Between Patterns of Alcohol Consumption and Gastrointestinal Symptoms Among Patients With Irritable Bowel Syndrome. The American Journal of Gastroenterology, 108(2), 270–276. https://doi.org/10.1038/ajg.2012.414
Stefanov, O. V. (2001). Doklinichni doslidzhennia likarskykh zasobiv. Kyiv: Avitsena, 528.
Tattis, A., Zupanecz`, I. A., Shebeko, S. K., Otrishko, I. A., Grinczov, Ye. F. (2016). Liky` Ukrayiny` Plyus, 3 (28), 63–67.
Swanson, G. R., Sedghi, S., Farhadi, A., & Keshavarzian, A. (2010). Pattern of alcohol consumption and its effect on gastrointestinal symptoms in inflammatory bowel disease. Alcohol, 44(3), 223–228. https://doi.org/10.1016/j.alcohol.2009.10.019
CHOI, J.-S., KIM, J. W., CHO, H.-R., KIM, K.-Y., LEE, J.-K., SOHN, J. H., & KU, S.-K. (2014). Laxative effects of fermented rice extract in rats with loperamide-induced constipation. Experimental and Therapeutic Medicine, 8(6), 1847–1854. https://doi.org/10.3892/etm.2014.2030
Wintola, O. A., Sunmonu, T. O., Afolayan, A. J. (2010). The effect of Aloe ferox Mill. in the treatment of loperamide-induced constipation in Wistar rats. BMC gastroenterology, 10, 95. https://doi.org/10.1186/1471-230x-10-95
Drogovoz, S. M., Borody`na, T. V., Dery`medvid`, L. V. (1998). Liky`, 5, 32–35.
Nair, A. B., Jacob, S. (2016). A simple practice guide for dose conversion between animals and human. Journal of basic and clinical pharmacy, 7(2), 27–31. https://doi.org/10.4103/0976-0105.177703
Gnjidic, D., Tinetti, M., Allore, H. G. (2017). Assessing medication burden and polypharmacy: finding the perfect measure. Expert review of clinical pharmacology, 10(4), 345–347. https://doi.org/10.1080/17512433.2017.1301206
Sagar, L., Sehgal, R., & Ojha, S. (2005). Evaluation of antimotility effect of Lantana camara L. var. acuelata constituents on neostigmine induced gastrointestinal transit in mice. BMC Complementary and Alternative Medicine, 5(1), 18. https://doi.org/10.1186/1472-6882-5-18
Khalafian, A. A. (2007). STATISTICA 6. Statisticheskii analiz dannykh. Uchebnik. Moscow: OOO «Binom-Press», 512.
Levitt, D., & Levitt, M. (2018). A model of blood-ammonia homeostasis based on a quantitative analysis of nitrogen metabolism in the multiple organs involved in the production, catabolism, and excretion of ammonia in humans. Clinical and Experimental Gastroenterology, 11, 193–215. https://doi.org/10.2147/ceg.s160921
Deacon, R. M. (2006). Housing, husbandry and handling of rodents for behavioral experiments. Nature Protocols, 1 (2), P. 936–946. https://doi.org/10.1038/nprot.2006.120.
Downloads
Published
Issue
Section
License
Copyright (c) 2019 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
