The study of the pharmacological action of the dry extract from Prunus domestica fruits on the model of comorbid functional constipation in the combined alcoholic liver damage in rats

Authors

  • Bashar Jabbar Ali Al-Sahlanee National University of Pharmacy
  • D. V. Litkin National University of Pharmacy
  • I. V. Senyuk National University of Pharmacy

DOI:

https://doi.org/10.24959/167342

Keywords:

Prunes Domestica fruits, hepatoprotectors, comorbid fuctional constipation, subacute liver damage, urea, ALT

Abstract

The search for the most effective hepatoprotective agents is an urgent problem of modern medicine. As a rule, crude plant is promising object for the study of medicinal properties. Based on the literature data, we are interested in the horticultural crops Prunus domestica, the family Rosaceae, which is quite cultivated and attracts attention to its pharmacoeconomic component.

The purpose of the study. In previous experimental studies, the Prunus domestica extract, containing fibers, was found to have laxative, hepatoprojector and probiotic effects. The foregoing provided an opportunity to study the Prunus domestica extract, containing fibers for activity in the comorbidization of several relevant pathologies and to experimentally substantiate the prospect of its application as a means of choice for constipation in patients with pathologies of the digestive system.

Materials and methods. The object of research was a dry extract from Prunus domestica fruits, containing fibers. The experiment was carried out in stages. At the first stage, the animals caused experimental subacute alcoholic liver damage. In the second stage of the experiment, the animals were modeled with functional constipation. During the course of subacute hepatosis and during the second phase of the experiment, the test extract was injected into the animal at a dose of 200 mg/kg and the reference drug was hepatoprotector Silybor at a dose of 25 mg/kg. In the second phase of the study, the laxative agent Senadexin at a dose of 14 mg/kg was added to the therapy. All substances were injected into the body of the experimental animals orally.

To evaluate the motor activity of the intestine, Sagar et al, modified by Choi et al., was used.

Motor activity of the small intestine was determined according to the corresponding calculation formula.

The functional state of the liver was evaluated according to the biochemical parameters in the blood serum: the total protein, urea and ALT activity.

Results. An analysis of experimental data showed that the test extract exhibits a moderate laxative effect.

In animals of the intact group, the relevant signs (pronounced laxative effect) indicate the development of diarrhea, which is not an adequate solution to the development of constipation.

With the use of the extract from Prunus domestica fruits during the whole period of the experiment, normalization of all indicators that characterize the intestinal motility was observed.

Analysis of experimental data showed that the investigated phytoobject has a soft laxative effect, mainly through the improvement of intestinal motility.

In the study of the functional state of the liver on the introduction of the investigated extract, positive changes in the content of markers of liver damage (decrease in urea concentration and decrease in the activity of ALT in animal serum) were detected in comparison with the control group of animals.

Conclusions. The obtained experimental data on the study of the correlation mechanisms of hepatoprotective and laxative activity of the extract from Prunus domestica fruits containing fibers indicate that there is a soft laxative effect that is realized with the help of intensifying intestinal motility, as well as the presence of pronounced hepatoprotective action. The indicated effects of the investigated extract were revealed when used in the treatment of constipation on experimental subacute liver damage, while not losing out to the studied effects in the complex application of hepatoprotective and laxative comparisons.

The key conclusion is that the test extract, as opposed to the reference drug, does not cause signs of diarrhea in its laxative effect, which can be a distinctive, positive feature in further clinical studies.

This herbal object can be promising and rational in application with single-stage complex therapy, such as hepatoprotective and laxative agent in the treatment of comorbid conditions in gastroenterology associated with diseases of the liver and congestive intestinal phenomena.

Author Biographies

Bashar Jabbar Ali Al-Sahlanee, National University of Pharmacy

postgraduate student of the Department of Biological Chemistry

D. V. Litkin, National University of Pharmacy

Head of the Central Scientific-Research Laboratory

I. V. Senyuk, National University of Pharmacy

PhD (Doctor of Philosofy), associate professor department of biological chemistry

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Published

2019-05-31

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Section

Pre-clinical studies of new drugs