The study of the antimicrobial activity of a soft dosage form with the antiviral effect

Authors

  • V. I. Hrytsenko National University of Pharmacy
  • L. S. Kienko National University of Pharmacy
  • L. O. Bobrytska National University of Pharmacy

DOI:

https://doi.org/10.24959/cphj.19.1491

Keywords:

antimicrobial activity, acyclovir, miramistin, soft dosage form

Abstract

Nowadays there is a tendency towards the spread of viral diseases that cause significant complications to human health. Therefore, the question of creating new drugs that have a high antiviral effect is topical.

Aim. To study the antimicrobial activity of the pharmaceutical composition with acyclovir and miramistin in a soft dosage form with the antiviral effect.

Materials and methods. The objects of the study were model samples of the soft dosage form containing acyclovir and miramistin in the concentrations of 0.25 % (sample 1), 0.5 % (sample 2), 0.75 % (sample 3). Staphylococcus aureus АТСС 25293, Bacillus subtilis АТСС 6633, Escherichia coli АТСС 25922 and Candida albicans АТСС 885/653 were used as test cultures. The antimicrobial activity of the experimental samples was studied in vitro by the agar diffusion method.

Results and discussion. According to the results of the studies it has been found that all samples studied have a wide range of the antimicrobial action in relation to the test strains used (the diameter of the growth inhibition zones of test cultures is 13-26 mm). It has been proven that the culture of the test strain Staphylococcus aureus АТСС 25293 has a higher sensitivity to samples 2 and 3.

Conclusions. The studies of the antimicrobial activity of the soft dosage form with the antiviral effect in relation to different cultures of microorganisms have been conducted, and the optimal concentration of the active substance – miramistin, 0.5 %, has been experimentally substantiated.

Author Biographies

V. I. Hrytsenko, National University of Pharmacy

Doctor of Pharmacy (Dr. habil), professor of the Department of Industrial Technology of Drugs

L. S. Kienko, National University of Pharmacy

postgraduate student of the Department of Industrial Technology of Drugs

L. O. Bobrytska, National University of Pharmacy

Doctor of Pharmacy (Dr. habil), professor of the Department of Industrial Technology of Drugs

References

Dmitriyevskiy, D. I., Bobritskaya, L. A. (2009). Zbirnik naukovih prats spivrobitnikiv NMAPO im. P. L. Shupika (Ukraine),18 (3), 335–340.

Rubleva, O. V. (2012). Rus. med. zhurn. 20, (11), 571–574.

Kazantseva, I. A., Kzantseva, N. N. (2016). Vestnik VolgGMU, 4 (60), 96–98.

Korovina, A. N., Kukhanova, M. K., Kochetkov, S. N. (2013). BIOTECHNOLOGIAACTA, 6(4), 78–85.

Celum, C, Wald, A, Lingappa, JR, Magaret, AS, Wang, RS. (2010). Acyclovir and transmission of HIV-1 from persons infected with HIV-1 and HSV-2. The new England journal of medicine, 362 (4), 427 – 439. https://doi.org/10.1056/NEJMoa0904849

Dunayevskiy, A. M., Kirichenko, I. M. (2013). Poliklinika, 5, 6–12.

Kirichenko, I. M. (2013). Poliklinika, 6, 100–102.

Barinskiy, I. F., Alimbarova, L. M., Kirichenko, N. A., Svistov, V. V., Smirnov, I. V., Krivoshein, YU. S. (2012). Dermatovenerologiya i dermatokosmetologiya, 2, 28–31.

Volianskyi, Yu. L., Hhrytsenko, I. S., Shyrobokov, V. P. (2004). Vyvchennia spetsyfichoi aktyvnosti protymikrobnykh likarskykh zasobiv. Kyiv, 38.

10. Derzhavna Farmakopeia Ukrainy. 2-e vyd., 1. (2015). Kharkiv: Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv, 1128.

Downloads

Published

2019-05-31

Issue

Section

Pre-clinical studies of new drugs