The study of the subchronic toxicity of the cream with cerium dioxide nanoparticles in cutaneous application in rabbits




cerium dioxide nanoparticles, subchronic toxicity, non-clinical studies, cutaneous application, Chinchilla rabbits


The development of photoprotectors with cerium dioxide nanoparticles (CDN) for prevention of sunburns and skin cancer is expedient.

Aim. To study the subchronic toxicity of the cream with CDN in cutaneous application in rabbits.

Materials and methods. The cream with 0.25 % CDN in the doses of 0.06, 0.18, and 0.60 g/kg was applied cutaneously to Chinchilla rabbits for 90 days. The toxicity was assessed by the general state, behavior of animals, the body mass dynamics, and blood hematological and biochemical indices.

Results. The repeated cutaneous application of the cream with CDN in the doses of 0.06, 0.18, and 0.60 g/kg in rabbits did not cause significant changes in the general state and behavior of animals, consumption of food and water, the body mass dynamics, and blood hematological (hemoglobin level, erythrocyte, leukocyte, and thrombocyte count, leukogram) and biochemical (total protein, albumin, glucose, cholesterol, potassium, sodium, and chloride levels, ALT and AST activity) indices. After 1 month of application of the cream in the dose of 0.60 g/kg the glucose level was 44.5 % higher than in the intact control group, but in 3 months of the experiment differences in this parameter disappeared.

Conclusions.The data obtained concerning the safety of the cream with CDN determined the expediency of further non-clinical studies of this photoprotector.


Author Biographies

G. V. Zaychenko, "Bogomolets National Medical University"

head of the Department of Pharmacology Bogomolets National Medical University

O. A. Pokotylo, "Bogomolets National Medical University"

teaching assistant of the Department of Pharmacology

N. S. Nikitina, "SOE "State Scientific Centre of Drugs and Healthcare Products"



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