DOI: https://doi.org/10.24959/cphj.19.1482

Dеvelopment of the analytical diagnostics of atomoxetine poisonings

S. A. Karpushyna, S. V. Baiurka, L. Yu. Tomarovska

Abstract


Atomoxetine is one of the main drugs used for the treatment of attention-deficit/hyperactivity disorder. The drug has repeatedly been the cause of fatal poisonings. Data on atomoxetine determination in the biological material are missing in the literature.

Aim. To determine the recovery of the methods generally accepted in chemicotoxicological analysis for drug isolation from the biological material with regard to atomoxetine.

Materials and methods. The study was performed with the model animal liver samples containing the drug under research. The detection and quantitative determination of the drug in the extracts was performed using thin-layer chromatography, color reactions and UV spectrophotometry.

Results. The recovery of the drug isolation was of 31.6 ± 3.0 % according to A. A Vasylieva’s method, 25.6±2.9 % according to Stas-Otto’s method, and 26.8 ± 2.8 % according to V. Ph. Kramarenko’s method. The limits of detection (LOD) and quantitative determination (LOQ) of the UV-spectrophotometric method for determination of atomoxetine in the biological material depending on the sample preparation method were determined. The LOD and LOQ values were 5.3 and 16.2 μg/ml (using A. A. Vasylieva’s method) and 2.3 and 7.1 μg/ml (using V. Ph. Kramarenko’s method), respectively. These values exceeded the corresponding values obtained for the standard solutions of atomoxetine, they were of 1.8 and 5.6 μg/ml, respectively. The LOD and LOQ values corresponding to isolation by Stas-Otto’s method were of 1.7 and 5.3 μg/ml, respectively.

Сonclusions. Isolation according to Stas-Otto’s method has provided the highest selectivity of the UV-spectrophotometric method for atomoxetine determination in the biological material with regard to the matrix components.


Keywords


atomoxetine; general isolation methods; thin-layer chromatography; UV-spectrophotometry

References


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GOST Style Citations


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