The effect of nanochromium citrate on the osmotic resistance of erythrocyte membranes in rats with the experimental diabetes mellitus

Authors

DOI:

https://doi.org/10.24959/cphj.17.1438

Keywords:

erythrocyte membranes, nanochromium citrate, experimental diabetes mellitus

Abstract

Diabetes mellitus (DM) is a serious medical and social problem because of its wide spread in the world and a steady tendency to increase the number of patients.

Aim. To study the effect of nanochromium citrate on osmotic resistance of erythrocyte membranes of animals with diabetes mellitus.

Materials and methods. The study was performed on 18-month laboratory male rats. Diabetes mellitus was caused by dexamethasone (0.125 mg/kg, subcutaneously during 14 days). Nanochromiun citrate (NCC) was introduced into the stomach (0.01 mg/kg during 14 days). Permeability of erythrocyte membranes (the erythrocyte osmotic stability) was detected by the erythrocyte resistance in the mixture of isotonic solutions of urea and sodium chloride with their different volumetric composition. The osmotic hemolysis degree (%) was measured by photocolorimetry in relation to the optic density of the standard urea solution. The erythrocyte membrane osmotic resistance (EMOR) – (% of hemolyzed cells) was compared with the similar index in the control intact animals. The glucose level in the blood was measured by a glucometer.

Results. In animals with the experimental DM in addition to the elevated glucose level in the blood to 14.2 ± 0.4 mmol/L the percentage of hemolyzed erythrocytes increased by 8.2-2.3 times compared to the control according to the increased concentration of urea in the solution. The increased permeability of erythrocyte membranes resulting in the reduced osmotic resistance of erythrocytes and their hemolysis is indicative of lipid peroxide oxidation (LPO) activation and decrease in activity of the antioxidant defense system in animals with DM. Introduction of NCC in DM reduced the glucose level in the blood to 6.6±0.4 mmol/L and increased EMOR considerably, and it indicates the presence of NCC membrane-protective properties.

Conclusions. In case of the experimental (dexamethasone) DM the erythrocyte membrane osmotic resistance decreases, destructive processes are activated, resulting in their accelerated destruction. NCC decreases the glucose level in the blood of animals with DM and increases osmotic resistance of erythrocyte membranes.

Author Biographies

K. V. Sadogurska, Bukovinian State Medical University

teaching assistant of the Department of Pharmacy

R. B. Kosuba, Bukovinian State Medical University

Doctor of Medicine (Dr. habil.), professor of the Department of Pharmacology

V. G. Zeleniuk, Bukovinian State Medical University

Candidate of Pharmacy (PhD), teaching assistant of the Department of Pharmacy

References

Tkachenko, V. I., Vydyborets, N. V., Kovalenko, O. F. (2013) Zdobutky klinichnoi i eksperymentalnoi medytsyny – Achievments of clinical and experimental medicine, 2, 177 – 182.

Ferents, I. V., Brodiak, I. V., Liuta, M. Ya. et al. (2014). Fiziolohichnyi zhurnal, 60 (4), 70 – 79.

Pankiv, V. I. (2015). Klinichna endokrynolohiia ta endokrynna khirurhiia – Clinical endocrinology and endocrine surgery, 2 (50), 58 – 63.

Pechova, A. (2007). Chromium as an essential nutrient: a review. Veterinarni Medicina, 52 (1), 1 – 18.

Kosinov, M. V., Kaplunenko, V. H. (2008). Sposib otrymannia akvakhelativ nanometaliv «Eroziino–vybukhova nanotekhnolohiia otrymannia akvakhelativ nanometaliv». Patent № 29856 UA, MPK B01J 13/00, B82B 3/00; declared 25.10.2007; published 25.01.2008, № 2.

Iskra, R. Ya., Svarchevska, O. Z., Maksymovych, I. Ya. (2012). Visnyk problem biolohii i medytsyny – Bulletin of problems in biology and medicine, 2 (2 (93)), 32 – 35.

Bondar, T. P., Kozinets, H. I. (2002). Klinichna laboratorna diahnostyka, 12, 22 – 34.

Kamyshnikov, V. S. (2000). Spravochnik po kliniko–biokhimicheskoi laboratornoi diagnostike. Minsk: Belarus, 495.

Moiseenko, V. A., Antonenko, L. I., Arshynnikova, L. L. et al. (2007). Krymskii terapevticheskii zhurnal, 2 (2), 103 – 106.

Hubskyi, Yu. I., Bukhtiarova, T. A., Horhoshko, H. H. et al. (2012). Medychna khimiia, 14 (4), 5 – 11.

Sadohurska, K. V. (2016). Potentsiini mozhlyvosti hipohlikemichnoi dii nanochastynok khromu, 1 (26). Kyiv, 113 – 114.

Stefanov, O. V. (2001). Eksperymentalne vyvchennia novykh hipohlikemichnykh zasobiv. Kyiv: Avitsenna, 396 – 408.

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Published

2017-12-11

Issue

Vol. 21 No. 4 (2017)

Section

Pre-clinical studies of new drugs

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