Platelet aggregation under the action of drugs of glucosamine hydrochloride and acetylsalicylic acid in acute cold injury

Authors

  • Ye. V. Bondarev National University of Pharmacy, Ukraine
  • S. Yu. Shtrygol National University of Pharmacy, Ukraine
  • I. A. Zupanets National University of Pharmacy, Ukraine
  • I. A. Otrishko National University of Pharmacy, Ukraine

DOI:

https://doi.org/10.24959/cphj.17.1411

Keywords:

cold injury, glucosamine hydrochloride, acetylsalicylic acid, platelet aggregation, frigoprotective action

Abstract

General and local cooling is a common factor of the effect of the environment on the human body. Exposure to cold can act as a damaging factor causing development of the destructive processes with the various degree of severity in tissues. It causes a complex reaction in the organism, which functioning in conditions of hypothermia greatly depends on the state of the blood system, including its rheological properties.

Aim. To determine the effect of the drugs studied on hemostasis, including platelet aggregation under conditions of acute cold injury.
Materials and methods. The study was performed in summer on white rats male weighing 280-320 g (24 rats); they were kept in standard vivarium conditions in accordance with the GLP rules.
Results. The state of platelet aggregation and the rectal temperature after acute cold injury (general cooling) in rats has been studied. When using “Glucosamine-C BCPP” and glucosamine hydrochloride the rectal temperature significantly increased in relation to the control pathology group, it indicated the improved condition of the animals. Acetylsalicylic acid (ASA) caused death of 50 % of the rats and did not affect the temperature decrease. Cold injury was accompanied by a significant (1.7 times) increase in the extent of ADP- and epinephrine-induced platelet aggregation, and the speed increased by 1.6-1.9 times. “Glucosamine-C BCPP” and glucosamine hydrochloride significantly reduced the intensity of ADP-induced aggregation, not being inferior ASA, and the rate of this process was at the trend level.
Conclusions. In contrast to the moderate antiplatelet effect of “Glucosamine-С BCPP” and glucosamine hydrochloride on the model of adrenaline-induced aggregation ASA caused an excessive effect with 1.5 times reduction in the intensity of aggregation compared to the intact control. This fact indicates the danger of bleedings and confirms the benefits of drugs of glucosamine as frigoprotective agents.

Author Biographies

Ye. V. Bondarev, National University of Pharmacy

Candidate of Pharmacy (Ph.D.), associate professor, associate professor of the Pharmacology Department

S. Yu. Shtrygol, National University of Pharmacy

Doctor of Medicine (Dr. habil.), professor, head of the Pharmacology Department

I. A. Zupanets, National University of Pharmacy

Doctor of Medicine (Dr. habil.), professor, head of the Clinical Pharmacology and Clinical Pharmacy Department

I. A. Otrishko, National University of Pharmacy

Candidate of Pharmacy (Ph.D.), associate professor, associate professor of the Clinical Pharmacology and Clinical Pharmacy
Department

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Published

2017-03-20

Issue

Vol. 21 No. 1 (2017)

Section

Pre-clinical studies of new drugs

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