The immunobiological assessment of the antibody titre in treating candidiases by antigens of C. Albicans fungi

M. V. Rybalkin, N. I. Filimonova, O. P. Strilets, L. S. Strelnikov

Abstract


Every year candidal infections affect a great number of the population, and annually the number of patients is growing around the world. At the premises of the National University of Pharmacy the authors have developed the method of cell disintegration of Candida albicans fungi. For this purpose ultrasonic disintegration at the wavelength of 22 kHz and exposure of 15 min was used. Then there was filtration through a «Vladipore» membrane MFA-MA No.3 providing separation of the biological material with the size of 10 kDa. The aim of our work was the immunobiological assessment of the antibody titres in treating candidiases with the cell-associated antigens of C. albicans fungi. The immunological action of the cell-associated antigens of C. albicans fungi in treating candidiases was determined in mice. The mice were preliminary infected with the candidal infection. In 5 days after infection the cell-associated antigens of C. albicans fungi under research with the protein concentration of 3 mg/ml in the volume of 0.2 ml were injected intramuscularly to mice twice with an interval of 14 days. Nothing was given to the animals of the control group. In 14 days the protective functions of the animals’ body were determined by the titres of the specific antibodies of C. albicans using enzyme-linked immunoassay (ELISA). When determining the antibody titre after the second injection of the cell-associated antigens of C. albicans fungi it has been found that the antibody titre is in the range of 1:1600 – 4000. The antibody titre in animals of the control group was within 1:400 – 1:1000. Based on the data obtained it may be concluded that the antigens studied have shown the pronounced immunological properties in the experiment in animals.

Keywords


candidiasis; antigen; vaccine; immunity; therapy; antibody titre

References


Голубка О.В. // Annals of Mechnikov Institute. – 2011. – №2. – С. 51-59.

Петров Р.В., Хаитов Р.М. Иммуногены и вакцины нового поколения. – М.: ГЭОСТАР-Медицина, 2011. – 608 с.

Cassone A., Casadevall A. // Curr. Opin. Microbiol. – 2012. – Vol. 15. – P. 1-7.

Carvalho A., Cunha C. // Front. Microbiol. – 2012. – Vol. 3. – P. 1-9.

De Bernardis F. et al. // Vaccine. – 2012. – № 30. – P. 4490-4498.

Diekema D., Arbefeville S., Boyken L. et al. // Diagn. Microbiol. Infect. Dis. – 2012. – №73. – P. 45-48.

Edwards J.E. // Med. Microbiol. – 2012. – №61. – P. 895-903.

Han Y., Rhew K.Y. // Arch. Pharm. Res. – 2012. – №35. – P. 2021-2027.


GOST Style Citations






DOI: https://doi.org/10.24959/cphj.15.1333

Abbreviated key title: Klìn. farm.

ISSN 2518-1572 (Online), ISSN 1562-725X (Print)