The immunomodulatory effect of Asperula odorata L. and Asperula humifusa m. Bieb. Besser dry extracts

Authors

  • N. V. Kashpur SI «Institute of microbiology and immunology n.I.I.Mechnikov NAMS», Ukraine
  • N. S. Yurchenko National University of Pharmacy, Ukraine
  • T. V. Ilyina National University of Pharmacy, Ukraine
  • A. M. Kovalyova National University of Pharmacy, Ukraine
  • O. V. Goryacha National University of Pharmacy, Ukraine
  • M. V. Smilyanska SI «Institute of microbiology and immunology n.I.I.Mechnikov NAMS», Ukraine
  • S. D. Peremot SI «Institute of microbiology and immunology n.I.I.Mechnikov NAMS», Ukraine

DOI:

https://doi.org/10.24959/cphj.15.1323

Keywords:

Asperula odorata, Asperula humifusa, phagocytic index, phagocytic number

Abstract

The research presents results of in vitro studies of the effect of dry extracts of Asperula odorata and Asperula humifusa on the functional activity of macrophages and their hematogenous precursors. It has been found that the extracts studied reveal a dose-dependent stimulatory effect on the transformation and phagocytic activities of macrophages and their mononuclear precursors. The Asperula humifusa dry extract exhibits a more expressed immunomodulatory effect in the dose range from 5 to 100 μg/ml. When using the extracts studied in the dose of 100 μg/ml the maximal stimulation of the functional activity of immune competent cells has been observed. Therefore, the extract of Asperula odorata increases the transformation activity of mononuclear cells by 57%, the phagocytic index is 46% and the phagocytic number is 54% compared to the control. The extract of Asperula humifusa increases the transformation activity by 64%, the phagocytic index is 44% and the phagocytic number is 56%. The research results confirm opportunities of using the given dry extracts with the purpose of immunopathological states correction.

References

Барнаулов О.Д. Фитотерапия больных бронхолегочными заболеваниями. – С.Пб.: Изд-во Н-Л, 2008. – 304 с.

Гарник Т.П., Фролов В.М., Пересадин Н.А. // Укр. мед. альманах. – 2008. – Т. 11, №5. – С. 50-56.

Гарник Т.П., Фролов В.М., Пересадин Н.А. // Укр. мед. альманах. – 2008. – Т. 11. №6. – С. 53-57.

Дранник Г.Н. Клиническая иммунология и аллергология. – 2-е изд. – М.: МИА, 2006. – С. 193-202.

Киселева Е.П., Цвейбах А.С., Гольдман Е.И., Пигарева Н.В. // Иммунол. – 1985. – №1. – С. 76-78.

Лапач С.Н., Чубенко А.В., Бабич П.Н. Статистические методы в медико-биологических исследованиях с использованием Ехсеl. – К.: Морион, 2000. – 320 с.

Никитин В.М. Справочник методов иммунологии. – Кишинев: Штиинца, 1982. – 304 с.

Пат. №50189 Україна. МПК А 61 К 36/185, А 61 Р 31/00, А 61 Р 37/00 Лікувально-профілактичний засіб з імуномоделюючою дією, активний у відношенні вірусу грипу / М.В.Іщенко, М.Л.Сятиня. – Заявка № u200913076. Заявл.: 15.12.2009. Опубл.: 25.05.2010. – Бюл. №10.

Столяров И.Д. Иммунодиагностика и иммунокоррекция в клинической практике. – С.Пб.: Сотис, 2003. – 176 с.

Фролов В.М., Кузнєцова Л.В., Пересадін М.О., Соцька Я.А. // Укр. мед. часопис. – 2008. – №5 (67). – С. 119-124.

Фролов В.М., Пересадин Н.А., Пшеничный И.Я. // Лаборат. дело. – 1990. – №9. – С. 27-29.

Alamgir M., Uddin S.J. // Ethnomedicine: A Source of Complementary Therapeutics. – 2010. – №4. – Р. 227-244.

Kvatchoff V.G. Application de subculture de leycocytes en test-systeme devalution immunomodule de vims in vitro // Abstracts of IV International Congress Cell Biology. – Montreal (Canada). – 1988. – P. 205.

Patil V.V., Bhangale S.C. // Intern. J. of Pharmacy and Pharmac. Sci. – 2010. – №2 (4). – Р. 97-99.

Salem M.L. // Intern. Immunopharmacol. – 2005. – №5. – Р. 1749-1770.

Downloads

Published

2015-03-10

Issue

Vol. 19 No. 1 (2015)

Section

Pre-clinical studies of new drugs

License

Copyright (c) 2015 National University of Pharmacy

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).