The stimulating effect of interleukin-33 on proliferation of the primary human lung fibroblasts

O. A. Bocharov

Abstract


Interleukin (IL)-33 is a multifunctional cytokine that belongs to the IL-1 cytokine family and expressed by multiple organs and cell types. Recent studies have showed that IL-33 plays an etiological role in several fibrotic disorders and may be involved in the pathogenesis of chronic respiratory diseases. It has been reported that IL-33–induced cutaneous fibrosis is associated with the increased fibroblast proliferation and altered expression of extracellular matrix-modifying genes. However, the role of IL-33 in regulating of functions of lung fibroblasts remains unclear. In the present study we examined the effect of IL-33 on proliferation of human lung fibroblasts. Five primary lines of normal adult human lung fibroblasts were cultured for 3-7 days in the presence of increasing concentrations of IL-33. We have observed that normal human lung fibroblasts responded in a dose-dependent manner to treatment with recombinant human IL-33 by increasing proliferation rates 1.5- to 2.3 fold compared to the non-stimulated control. The maximum effect of IL-33 on fibroblast proliferation was observed in the cytokine concentrations range from 2 ng/ml to 100 ng/ml. These results suggest that IL-33 may play an important role in the regulation of the human lung fibroblast proliferation. Human lung fibroblasts activated by IL-33 may act as effector cells not only in the pathogenesis of lung diseases, but also in lung remodeling processes.


Keywords


interleukin-33; human lung fibroblasts; proliferation

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References


Granne I., Southcombe J.H., Snider J.V. et аl. // PLoS One. –2011. – Vol. 6, №9. – P. 1.

Liew F.Y., Pitman N.I., McInnes I.B. // Nat. Rev. Immunol. –2010. – Vol. 10, №2. – P. 103-110.

Miller A.M. // J. Inflamm. (Lond). – 2011. – Vol. 8, №8 – P. 22.

Nabe T. // J. Pharmacol. Sci. –2014. – Vol. 126, №9.

Nishida A., Andoh A., Imaeda H. et аl. // Gut. – 2010. – Vol. 59, №4. – P. 531-541.

Oboki K., Ohno T., Kajiwara N. et аl. // Allergol. Int. – 2010. – Vol. 59, №2. – P. 143-160.

Préfontaine D., Nadigel J., Chouiali F. et аl. // J. Allergy Clin. Immunol. – 2010. – Vol. 125, №3. – P. 752-754.

Rankin A.L., Mumm J.B., Murphy E. et аl. // J. Immunol. –2010. – Vol. 184, №3. – P. 1526-1535.

Roussel L., Erard M., Cayrol C., Girard J.P. // EMBO Reports. –2008. – Vol. 9, №10. – P. 1006-1012.

Schmitz J., Owyang A., Oldham E. et аl. // Immunity. – 2005. – Vol. 23, №5. – P. 479-490.

Yamamoto T. // Self Nonself. – 2011. – Vol. 2, №1. – P. 4-10.


GOST Style Citations






DOI: https://doi.org/10.24959/cphj.14.1326

Abbreviated key title: Klìn. farm.

ISSN 2518-1572 (Online), ISSN 1562-725X (Print)