DOI: https://doi.org/10.24959/cphj.14.1305

Experimental evaluation of the hydrochlorothiazide impact on the acid-base balance during complicated pregnancy in rats

E. L. Toryanik

Abstract


This article presents the results of the study of acid-base balance in pregnant white rats with experimental metabolic acidosis. The experiments have been conducted on nonlinear female rats with reproducible metabolic acidosis caused by daily, during a month, administration of ammonium chloride in the dose of 4 mg/kg. To assess intensity of disorders the standard indices of the acid-base status (ABS) and gas exchange of the blood of animals have been determined with the help of the blood gas analyzer. One of the tasks of our research was to study the effect of thiazide diuretics of hydrochlorothiazide on performance of the acid-base homeostasis in pregnant female rats on the background of metabolic acidosis reproduced as an experiment. It has been found that the ABS shift in pregnant female rats against the background of administration of ammonium chloride in the earlier gestational period (13 days) corresponds to the figure of subcompensated metabolic acidosis, becoming more intense at the later stages of gestation (19 days) in the compensated form. The dose-dependent effect of hypothiazide on ABS of pregnant female rats in terms of reproduced metabolic acidosis has been revealed. It has been shown that administration of hydrochlorothiazide in the dose of 20 mg/kg in late gestation in rats with metabolic acidosis does not have a significant negative impact on ABS, while increase of the dose of hydrochlorothiazide up to 40 mg/kg leads to decompensated acidosis. The results obtained experimentally substantiate the acceptance of hydrochlorothiazide use in certain doses during complicated pregnancy in late gestosis, against the background of hypertension and the threat to life in pregnant women.


Keywords


pregnancy; preeclampsia; metabolic acidosis; acid-base status; diuretics

References


Абрамченко В.В. Фармакотерапия гестоза. – С.Пб.: СпецЛит, 2010. – 477 с.

Венцковский Б.М., Запорожан В.Н., Сенчук А.Я. и др. Гестозы: Руковод. для врачей. – М.: Мед. информ. агентство, 2005. – 312 с.

Вихляева Е.М. // Акушерство и гинекол. – 2009. – №2. – С. 5-7.

Яковлєва Л.В., Зайченко Г.В., Ципкун А.Г. та ін. Доклінічне вивчення лікарських засобів, призначених для лікування плацентарної дисфункції: Метод. рекоменд. – К. : ДФЦ МОЗ України, 2009. – 59 с.

Кустаров В.Н., Линде В.А. Гестоз: патогенез, симптоматика, лечение. – С.Пб.: Гиппократ, 2012. – 125 с.

Машковский М.Д. Лекарственные средства. – 17-е изд., перераб., испр. и доп. – М.: Новая волна: Издатель Умеренков, 2011. – 1216 с.

Оганов Р.Г., Небиеридзе Д.В. Метаболические и сосудистые эффекты антигипертензивной терапии. – М.: Универсум Паблишинг, 2010. – С. 126-131.

Bayliss H., Chuchill D., Beevers M. et al. // Hypertens. Pregnancy. – 2012. – Vol. 21, №1. – P. 161-174.

Bowen R.S., Moodley J., Dutton M.F. et al. // J. Obstet. Gynaecol. – 2011. – Vol. 21, №6. – P. 563-569.

Cunningham F.G., Lindheimer M.D. // New End. – 2012. – Vol. 326, №9. – Р. 927-932.

Irons D.W., Baylis P.H., Batler T.J. et al. // Amer. J. Physiol. – 2007. – Vol. 273, №3. – Р. 483-487.

Lain K.Y., Roberts M. // JAMA. – 2010. – Vol. 287, №24. – P. 3183-3186.

Magee L.A. // Drug Safety. – 2011. – Vol. 24, №6. – P. 457-474.

Peters R.M., Flack J.M. // J. Obstet. Gynecol. – 2012. – Vol. 33, №2. – P. 209-220.

Piepsz A., Tondeur M., Ham H. et al. // Eur. J. Nucl. Med. And Mol. Imag. – 2012. – Vol. 39, №3. – Р. 295.

Redman C.W., Sargent I.L. // Gynecol. Obstet. Fertil. – 2011. – Vol. 29, №7-8. – P. 518-522.


GOST Style Citations






Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Abbreviated key title: Klìn. farm.

ISSN 2518-1572 (Online), ISSN 1562-725X (Print)