Screening of the antioxidant activity of extracts from Hedera helix leaves using the HPLC/ABTS method
DOI:
https://doi.org/10.24959/cphj.20.1533Keywords:
Hedera helix leaves, antioxidant activity, HPLC, screeningAbstract
Hedera helix is widely used as a remedy to treat the respiratory infections and cold accompanied with cough due to its anti-inflammatory effect. In addition, the antioxidant activity of its extracts has been confirmed. It is explained by the high content of flavonoids and phenolic acids among all phytochemicals of H. helix leaves. However, it remains uncertain which exactly components are responsible for the antioxidant activity and what is the best way to perform extraction.
Aim. To determine the antioxidant profile of different extracts from H. helix leaves using the in vitro HPLC method combined with the ABTS reagent.
Materials and methods. Extraction of H. helix leaves was conducted with different solvents (from 20 % methanol to 100 % methanol using an ultrasound bath); the method described in the Pharmacopeia was also used. A Waters chromatograph was used to determine the antioxidant profile.
Results. About 90 % of the components responsible for the antioxidant activity were determined using the HPLC method proposed. Among them, chlorogenic acid and 3,5-caffeoylquinic acid showed the highest activities. Other components, such as neochlorogenic acid, hyperoside and 3,4-caffeoylquinic acid were revealed as components with the antioxidant scavenging activities in H. helix lextracts.
Conclusions. The results obtained indicate that extracts from H. helix leaves possess the high antioxidant scavenging capacity. In addition, the in vitro HPLC method proposed can be used for the primary screening of components in the plant raw material.References
Obodozie O. O. (2012). Pharmacokinetics and drug interactions of herbal medicines: a missing critical step in the phytomedicine/drug development process.Readingsin Advanced Pharmacokinetics-Theory, Methods, and Applications. InTech. doi: https://doi.org/10.5772/33699.
Süleyman H., Mshvildadze V., Gepdiremen A., Elias R. (2003). Acute and chronic antiinflammatory profile of the ivy plant, Hedera helix, in rats. Phytomedicine, 10 (5), 370-374. doi: 10.1078/0944-7113-00260.
Medeiros JMR, Macedo M., Contancia J.P., Nguyen C., Cunningham G., Miles D. H. (2000). Antithrombin activity of medicinal plants of theAzores. J Ethnopharmacol, 72 (1-2), 157-165. doi: 10.1016/s0378-8741(00)00226-9.
Bezruk I., Kotvitska A., Korzh Iu., Materiienko A., Gubar S., Budanova L., Ivanauskas L., Vyshnevsky I., Georgiyants V. (2020). Combined Approach to the Choice of Chromatographic Methods for Routine Determination of Hederacoside C in Ivy Leaf Extracts, Capsules, and Syrup. Sci. Pharm., 88 (24). doi: https://doi.org/10.3390/scipharm88020024.
Directive 2010/63/EU Of The European Parliament And Of The Council of 22 September 2010 on the protection of animals used for scientific purposes. Available at: https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32010L0063&from=en.
Kolodeznaya T., Dobrova V. Ye. (2014). Alternative approaches of pathology’s modeling in pharmacological studies of medicines. Proceeding from Actual Questions Of Development оf New Drugs : Abstracts оf XX International Scientific And Practical Conference Of Young Scientists And Student (April 22-23, 2014). (p. 208). Kharkiv : Publishing Office.
BezrukI., Marksa M., Georgiyants V., Ivanauskas L., Raudone L. (2020). Phytogeographical profiling of ivy leaf (Hedera helix L.). Industrial Crops and Products., 154. doi: https://doi.org/10.1016/j.indcrop.2020.112713.
European Directorate for the Quality of Medicines and HealthCare. (2019). European Pharmacopoeia. 10th ed.Strasbourg.
Marksa M., Radušienė J., Jakštas V., Ivanauskas L. (2016). Development of an HPLC post-column antioxidant assay for Solidagocanadensis radical scavengers. Natural Product Research., 30 (5), 536-543. doi: https://doi.org/10.1080/14786419.2015.1027703.
Marksa, M., Zymone K., Ivanauskas L., Radušienė J., Pukalskas A., Raudone L. (2020). Antioxidant profiles of leaves and inflorescences of native, invasive and hybrid Solidago species. Industrial Crops and Products, 145. doi: https://doi.org/10.1016/j.indcrop.2020.112123.
Raudonis R., Bumblauskiene L., Jakstas V., Pukalskas A., Janulis V. (2010). Optimization and validation of post-column assay for screening of radical scavengers in herbal raw materials and herbal preparations. J. Chromatogr. A., 1217, 7690-7698. doi: https://doi.org/10.1016/j.chroma.2010.10.017.
Downloads
Published
Issue
Section
License
Copyright (c) 2020 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
